MacConkey Broth - BioReagent

Cat. No.: M1528475

CALIDAD & PUREZA BioReagent

Storage

Room temperature

Shipped In

Normal

Descripción general

For selective cultivation of intestinal bacteria

Especificaciones

Especificaciones y pureza

BioReagent

Condiciones de almacenamiento de almacenamiento

Room temperature

Enviado en

Normal

Grado

BioReagent

Product Line

Productos biológicos

Application Protocols

No antibody/assay protocols are applicable. The following is general, literature-based guidance for using MacConkey Broth; it is not item-specific and should be adapted to your SOPs. Always refer to the CoA/Spec Sheet for exact preparation instructions.

Example workflow (literature):

• Reconstitution: Dissolve the specified grams of dehydrated medium per liter of purified water (per CoA). Heat gently with stirring to aid dissolution if needed. Adjust pH as required by your method.
• Sterilization: Autoclave the solution using validated parameters (commonly 121 °C for ~15 min; adjust to load/volume per SOP). Allow to cool to handling temperature.
• Inoculation: Aseptically inoculate with test sample or control strains (e.g., lactose-fermenting E. coli as positive control; a Gram-positive organism to verify inhibition, per lab policy).
• Incubation: 30–37 °C for 18–48 h depending on method and organism.
• Assessment: Record turbidity and any indicator-based color changes. For confirmation, subculture onto MacConkey Agar or perform biochemical identification.
• Quality controls: Include sterility controls (uninoculated tubes) and growth promotion/indicative performance checks as part of routine QC.

Item-specific mass-per-liter, pH setpoint, and indicator presence: Not specified for this item; refer to CoA/Spec Sheet.

Biological Roles

MacConkey Broth plays a methodological role in microbiology by creating a defined selective and differential environment that probes microbial physiology.

• Selective pressure (literature): Bile salts and optionally crystal violet disrupt membranes or inhibit cell wall synthesis pathways preferentially in Gram-positive organisms, enriching for Gram-negative enterics adapted to bile-rich environments (e.g., intestinal flora).
• Differential metabolism: Lactose utilization via β-galactosidase/β-galactoside permease leads to acid production that lowers pH. With a suitable indicator present, lactose-positive organisms are distinguished from lactose-negative ones. Gas production may occur depending on organism and conditions.
• Nutritional support: Peptones/tryptone provide amino acids, peptides, vitamins, and growth factors supporting a broad spectrum of heterotrophs.
• Research utility: Enables studies on carbon utilization, membrane robustness against bile salts, and regulation of lactose operon systems in Enterobacterales. It is frequently used in enrichment steps preceding isolation on solid differential media (e.g., MacConkey Agar).

Note: Exact inhibitory strength and indicator response depend on the specific formulation and concentration of selective agents and dyes. Item-specific composition and pH: Not specified for this item; refer to CoA/Spec Sheet.

Buffer Applications

MacConkey Broth is a nutrient medium with selective agents, not a dedicated buffering solution. While prepared media possess a working pH (often around neutral in literature) and some buffering capacity due to phosphate and organic components, it is not typically used as a buffer in biochemical assays.

• Typical prepared pH: Approximately neutral (literature for standard formulations). Item-specific pH range: Not specified for this item; refer to CoA/Spec Sheet.
• If buffer capacity is critical for your assay, use a defined buffer system (e.g., phosphate, HEPES) and validate compatibility with the targeted microorganisms.

For electrophoresis, enzyme kinetics, or cell-free systems, dedicated buffers are recommended. For microbial culture, rely on the medium as provided and avoid uncontrolled pH alterations that could impact selectivity/differentiation.

Green Alternatives

Green chemistry considerations for culture media focus on waste minimization, energy use during sterilization, and supply-chain impacts rather than solvent replacement.

Potential improvements (general):

• Right-sizing and just-in-time preparation: Prepare only the volume needed to reduce disposal of unused sterile broth.
• Energy-efficient sterilization: Consolidate autoclave cycles and use validated minimum times consistent with sterility assurance to reduce utility consumption (without compromising biosafety).
• Recyclable packaging: Choose packaging options compatible with your institution’s recycling streams where available.
• Ready-to-use sterile media vs. dehydrated: Can reduce in-lab energy use and handling of powders but may increase shipping weight and packaging; assess tradeoffs.

Functional alternatives (application-driven):

• If the goal is presumptive coliform detection with minimal inhibitory dyes, Lauryl tryptose broth may offer simpler disposal profiles.
• For selective confirmation with comparable performance, Brilliant Green Bile (BGBB) or EC broth may be preferred per standard methods.

Tradeoffs:

• Dehydrated media minimize shipping mass and cold-chain needs (this product ships under normal conditions), but require on-site sterilization energy.

Item-specific environmental metrics or certifications: Not specified for this item; refer to CoA/Spec Sheet.

Pharmaceutical Uses

No therapeutic or clinical use is intended. For research use only.

Pharmacopeial/formulation context (general, non-clinical):

• MacConkey-type media are commonly used in pharmaceutical quality control laboratories for microbiological testing, including environmental monitoring, water testing, and raw material/finished product testing as per compendial guidance (e.g., growth promotion testing with specified strains). Specific compendial references vary by region and method; consult your quality system.
• Use cases include growth promotion tests (GPT) of culture media, selectivity verification, and method suitability studies where recovery of Gram-negative enteric bacteria and inhibition of Gram-positive organisms are required.

Regulatory notes:

• Any use in GMP environments requires qualification of each lot via GPT, sterility checks, and documentation traceability.
• Item-specific certifications, compliance statements, or validated organisms: Not specified for this item; refer to CoA/Spec Sheet.

Handling in QC labs:

• Prepare according to validated SOPs, record lot numbers, and document sterilization parameters. Dispose of used media as biohazardous waste following site procedures.

Physical Properties

As a dehydrated microbiological medium (complex mixture), MacConkey Broth lacks a single set of compound-level physical constants. Properties below reflect general, literature-level characteristics of typical MacConkey media and practical handling attributes; they are not item-specific specifications.

• Appearance (dehydrated): Typically free-flowing powder; color may range from light beige to pinkish depending on indicator content (literature). Item-specific: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Readily soluble in purified water to form a clear to slightly opalescent solution prior to sterilization (literature). Item-specific clarity after prep: Not specified for this item; refer to CoA/Spec Sheet.
• pH (at 25 °C): Typical prepared medium pH ~7.1 ± 0.2 (literature for many standards). Item-specific: Not specified for this item; refer to CoA/Spec Sheet.
• Density, melting/boiling point, refractive index, logP, pKa: Not applicable to this complex mixture; not determined.
• Typical working concentration: Commonly prepared at a defined grams-per-liter charge (e.g., on the order of a few tens of g/L; literature). Item-specific mass per liter: Not specified for this item; refer to CoA/Spec Sheet.

Note: Final properties depend on exact formulation, water quality, and sterilization conditions. Always verify against the product’s CoA/Spec Sheet.

Quality and Grades

• Grade/Purity: BioReagent

What BioReagent typically implies (general guidance):

• Suitable for biological and microbiological research applications where consistent growth performance and selectivity are required.
• Manufactured with attention to lot-to-lot reproducibility in nutrient content and inhibitory activity.
• Not intended for clinical diagnostics in human/animal care; for research use only (per product data).

Item-specific characteristics:

• Exact formulation, indicator content, inhibitory strength, and performance criteria (e.g., growth promotion and selectivity tests against defined ATCC strains) are critical but are not listed here. Item-specific performance specifications: Not specified for this item; refer to CoA/Spec Sheet.

Notes on stabilizers and additives:

• Dehydrated media generally contain no volatile stabilizers; moisture control is key to maintain free-flowing characteristics and performance. Any anti-caking agents or formulation nuances: Not specified for this item; refer to CoA/Spec Sheet.

Recommended verification (best practice):

• Perform growth promotion testing (GPT) with representative organisms (e.g., lactose-fermenting and non-fermenting Enterobacterales; Gram-positive controls for inhibition) upon receipt and periodically thereafter.
• Record lot numbers in all experiments for traceability.

Reaction and Applications

This product is a microbiological culture medium, not a chemical reagent for synthetic reactions. The relevant “applications” are microbiological.

Microbiological applications (literature/practice):

• Selective enrichment of Gram-negative enterics: Bile salts (and optionally crystal violet) suppress many Gram-positive organisms, enriching for Enterobacterales and related Gram-negative rods.
• Differentiation by lactose fermentation: Lactose-fermenters produce acid that changes the color of an included pH indicator (commonly neutral red in plate media). In broth, indicator response may be observed as a color change and/or turbidity differences depending on formulation.
• Quality control testing: Used in food/water/environmental microbiology and industrial hygiene laboratories for presumptive detection of coliforms and other lactose-fermenting Gram-negative bacteria (method-dependent).

Practical tips:

• Prepare with high-purity water; adjust pH if required by the standard method. Filter or clarify if necessary prior to sterilization.
• Sterilize via autoclave; cool to handling temperature before inoculation.
• Include positive controls (e.g., Escherichia coli) and negative controls (e.g., a Gram-positive strain) to verify selectivity and differential performance.
• For enrichment workflows, subculture onto differential/selective agar (e.g., MacConkey Agar) for colony-level confirmation.

Item-specific composition and indicator presence: Not specified for this item; refer to CoA/Spec Sheet.

Reaction Conditions

Chemical reaction conditions are not applicable. For microbiological culture, general incubation conditions (literature-level guidance) are provided for context; these are not item-specific specifications and should be adapted to your organisms and methods.

• Preparation: Dissolve the dehydrated medium in purified water at the specified grams-per-liter per your CoA/SOP; adjust pH if required by the method; sterilize (commonly by autoclaving).
• Inoculation: Introduce test organisms under aseptic conditions.
• Incubation: Typical temperatures for enteric bacteria are 30–37 °C depending on the test method; time frames often range 18–48 h (literature). Aeration/agitation may be applied for broth cultures to enhance oxygenation when appropriate.
• Readout: Observe turbidity and, if indicator is present, color change consistent with lactose fermentation. Confirm with streaking to differential agar or performing biochemical confirmation tests.

Item-specific prep concentration, pH, sterilization cycle, and readout criteria: Not specified for this item; refer to CoA/Spec Sheet and your validated SOP.

Safety and Handling

Hazard classification and GHS labeling are not provided for this specific item. Always consult the SDS for authoritative guidance.

• GHS classification, pictograms, signal word, H-statements: Not specified for this item; refer to SDS.
• General handling (literature/practice):
  • Avoid creating dust; dehydrated media may cause mechanical irritation to eyes, skin, and respiratory tract. Use in well-ventilated areas or a fume hood when weighing.
  • Wear appropriate PPE: lab coat, safety glasses/goggles, and nitrile gloves. Consider dust mask/respirator if airborne particulates may be generated (per risk assessment).
  • Avoid inhalation and contact with skin/eyes. Wash thoroughly after handling.
• Incompatibilities: Strong oxidizers and acids/bases may degrade organic components. Store away from moisture to prevent caking and microbial contamination (general practice).
• Thermal processing: Autoclaving is typically used for sterilization of prepared medium. Use suitable, pressure-rated vessels and follow institutional autoclave SOPs.
• Biohazard note: Once inoculated, the medium can contain pathogenic organisms. Treat all used broth as biohazardous; autoclave prior to disposal in accordance with local regulations.
• First aid (overview; defer to SDS):
  • Eye contact: Rinse cautiously with water for several minutes; remove contacts if present; seek medical attention if irritation persists.
  • Skin contact: Wash with soap and water.
  • Inhalation: Move to fresh air; seek medical advice if symptoms occur.
  • Ingestion: Rinse mouth; seek medical attention if unwell.

Solvent Selection

Solvent selection in the classical sense does not apply; MacConkey Broth is designed to be dissolved in water to make an aqueous culture medium.

• Required solvent: Purified/deionized water meeting microbiological media preparation standards (e.g., ASTM Type I/II, low microbial load). Water quality impacts clarity, pH, and selectivity.
• Miscibility: Fully water-soluble at the intended preparation concentration (literature). Do not use organic solvents.
• Sterilization approach: Typically sterilized by autoclaving as a complete solution. Some components in other media can be heat-labile; for standard MacConkey Broth, heat sterilization is standard in literature. Always follow your SOP.

When to choose MacConkey Broth vs alternatives (general):

• Choose MacConkey Broth for enrichment and presumptive detection of Gram-negative enteric bacteria with differentiation by lactose fermentation (acid production reflected by indicator response in many formulations).
• Consider alternatives for specific workflows:
  • Lauryl tryptose broth for presumptive coliform testing (MPN methods).
  • Violet Red Bile (VRB) broth/agar for coliform enumeration in foods.
  • BGBB (Brilliant Green Bile Broth) for confirmed coliform tests.
  • EC broth for thermotolerant coliforms/E. coli enrichment.

Item-specific preparation volume/concentration: Not specified for this item; refer to CoA/Spec Sheet.

Storage and Reconstitution

• Storage conditions (from Product Data): Room temperature. Protect from moisture and excessive heat. Keep container tightly closed to prevent caking and contamination. Shipped under normal conditions.

Reconstitution and sterilization (literature-level guidance; not item-specific):

• Water: Use purified/deionized water.
• Concentration: Prepare at the grams-per-liter charge specified on the product’s CoA/Spec Sheet. Item-specific concentration: Not specified for this item; refer to CoA/Spec Sheet.
• Dissolution: Add powder gradually with stirring; warm gently if needed to assist dissolution. Avoid overheating or localized scorching.
• pH: Adjust to the recommended setpoint for your method before sterilization if required. Item-specific pH: Not specified for this item; refer to CoA/Spec Sheet.
• Sterilization: Typically by autoclaving in suitable containers using validated parameters (e.g., 121 °C for an appropriate hold time per volume). Allow to cool before use.

Post-preparation storage (general):

• Store sterile broth at 2–8 °C if held for extended periods; minimize light exposure if indicator dyes are present. Observe recommended shelf-life from your SOP.
• Inspect for precipitation, contamination, or color changes prior to use; discard if compromised.

Re-freeze/thaw guidance: Not applicable; do not freeze dehydrated medium. Avoid repeated thermal cycling of prepared broth.

Structure and Identity

MacConkey Broth is a complex, non-defined microbiological culture medium formulated to selectively enrich Gram-negative enteric bacteria and differentiate lactose fermenters from non-fermenters. It is a mixture of organic and inorganic components (e.g., peptones, bile salts, lactose, dyes/indicators) and therefore does not have a single molecular structure.

• SKU: M1528475
• Product name: MacConkey Broth
• CAS: Not applicable (complex mixture)
• Molecular formula: Not applicable (complex mixture)
• Molecular weight: Not applicable (complex mixture)
• SMILES / InChI / InChIKey: Not applicable (mixture)

Structural features (general knowledge):

• Contains peptone/tryptone (complex peptides and amino acids) as nitrogen and growth factors.
• Lactose as fermentable carbohydrate to assess fermentation.
• Bile salts and often crystal violet provide selective pressure against Gram-positive organisms.
• pH indicator (e.g., neutral red in plate media; broth formulations may include an indicator depending on standard) to visualize acidification from lactose fermentation.

2D description in words:

• A heterogeneous blend of powders/granules; in solution (after dissolution and sterilization), forms a nutrient medium with selective agents and a pH indicator enabling colorimetric differentiation of lactose fermentation.

Item-specific identifiers or exact composition: Not specified for this item; refer to CoA/Spec Sheet.

Synthetic Utility

Not applicable. MacConkey Broth is a microbiological culture medium and is not used as a reagent or building block in chemical synthesis. It does not participate in named organic reactions, cross-couplings, or protection/deprotection strategies.

For users seeking chemical reagents with selective or indicator properties in synthesis, consider well-defined pH indicators, phase-transfer catalysts, or specific salts/acids/bases rather than complex media.

Target Specificity

This section typically applies to antibodies, enzymes, or probes with defined molecular targets. It is not applicable to a complex culture medium.

General note (literature): MacConkey media are designed to be selective for Gram-negative enteric bacteria and inhibitory to many Gram-positive organisms due to bile salts and optional crystal violet. They are differential for lactose fermentation when a suitable indicator is present.

Item-specific organism recovery/inhibition performance claims: Not specified for this item; refer to CoA/Spec Sheet or method validation data.