GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)1.0 mg/mL
mCherry is a fluorophore (a fluorescent molecule) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cells components. mCherry is a monomeric, fluorescent, photostable red dye that was isolated from an
Especies hospedadoras
Mouse
Especificidad
mCherry
Inmunógeno
Recombinant full length protein corresponding to mCherry
Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
Tipo de cadena ligera
kappa
SDS-PAGE
150.0 KDa
Método de purificación
Protein A purified
Concentración
1.0 mg/mL
Almacenamiento y envío
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
mCherry Mouse mAb (Ab169012) - Western Blot All lanes: mCherry Mouse mAb (Ab169012) at 1/10000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 45, 55 kDa Observed band size: 47, 55 kDa Exposure time: 60.0 s
mCherry Mouse mAb (Ab169012) - Flow Cytometry Overlay histogram showing HEK293T cells transfected with a mCherry tag using mCherry Mouse mAb (Ab169012) (Right panel). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (AF647) (Ab181952) was used as the secondary antibody. The mouse IgG (Ab170221) was used as an isotype control (Left panel).
mCherry Mouse mAb (Ab169012) – IF/ICC IF analysis of 293T cells transfected with a mCherry-DYKDDDDK tag using mCherry Mouse mAb (Red). The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with mCherry Mouse mAb (Ab169012) at 1.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (AF647) (Ab181952) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
Aplicación
Aplicación
Dilution info
IF/ICC
1:1000
WB
1/1000 - 1/20000
Flow Cytometry
1/200–1/5000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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