Elastic fibers are mainly distributed in the arterial wall, alveolar wall, and skin of the human body. They appear yellow when fresh and have strong refraction. Common elastic fiber staining methods include Gomori Aldehyde Fuchsin, Resorcinol-Fuchsin, Orcein, Victoria Blue, and Iron-Iodine Hematoxylin. Modified Weigert Elastic Fiber Staining Solution is mainly composed of Resorcinol-Fuchsin Staining Solution and modified Van Gieson (VG) Staining Solution. Resorcinol-Fuchsin Staining Solution is mainly used for elastic fiber staining and is also known as Weigert Resorcinol Fuchsin Staining Solution. Van Gieson staining is mainly used for collagen fiber staining. Its staining principle is related to the molecular size of anionic dyes and tissue permeability: Picric acid (PA) has the smallest molecular weight, followed by Ponceau and Fuchsin, while Light Green has the largest. After VG staining, muscle fibers appear yellow and collagen fibers appear red.
Modified Weigert Elastic Fiber Staining Solution is that resorcinol-fuchsin forms a lake complex with iron ions, and its free amino groups bind firmly to hydrogen bonds in elastic fibers, appearing black. Compared with the conventional Weigert resorcinol-fuchsin method, this modified method adds oxidation and bleaching steps. It is suitable for demonstrating various elastic fibers, with clearer staining, less fading, and longer storage time. This product can demonstrate changes of elastic fibers in skin tissue, such as elastic nevus, granuloma annulare, and scleroderma; demonstrate and evaluate endocardial and arterial lesions, and observe whether some lesions are accompanied by hyperplasia or destruction of elastic fibers. It can also identify tumor components such as elastofibroma, in which elastic fiber globules can be clearly seen after elastic fiber staining. This reagent is for research use only and not for clinical diagnosis or other purposes.
Product Components and Storage Conditions:
I1518582
| Component
| 5×50mL
| Storage
|
I1518582A
| Weigert Oxidant A
| 25mL
| 2-8℃. Store in the dark.
|
I1518582B
| Weigert Oxidant B
| 25mL
| RT
|
I1518582C
| Weigert Bleaching Agent
| 50mL
| RT
|
I1518582D
| Weigert Resorcinol-Fuchsin Staining Solution
| 50mL
| 2-8℃. Store in the dark.
|
I1518582E
| Acid Differentiation Solution
| 50mL
| RT
|
I1518582F
| Ponceau S Solution
| 5mL
| RT
|
I1518582G
| PA Solution
| 45mL
| RT
|
Immediately before use, mix equal volumes of Solution A and Solution B to prepare Weigert Oxidant. Do not prepare in advance.
Immediately before use, mix Solution F and Solution G at a ratio of 1:9 to prepare VG Staining Solution. Do not prepare in advance.
Materials Required (User-supplied):
1. 10% Formalin Fixative, graded ethanol, xylene or eco-friendly dewaxing clearing agent, neutral balsam
2. Staining jars, incubator
Protocol (For Reference Only):
1. Fix tissues in 10% formalin, followed by routine dehydration and embedding.
2. Cut 4‑μm sections, dewax with xylene or eco‑friendly dewaxing clearing agent, and hydrate to water.
3. Oxidize with freshly prepared Weigert Oxidant for 5 min, then rinse briefly with water.
4. Bleach with Weigert Bleaching Agent for 1–2 min, wash under running water for 2 min, then rinse briefly with 95% ethanol.
5. Place sections in a staining jar (capped) containing Weigert Resorcinol‑Fuchsin Staining Solution. Stain for 1–3 h at room temperature or 0.5–1 h at 56 °C.
6. Differentiate in Acid Differentiation Solution until no more stain elutes (about 2–3 s), then wash under running water for 10 min.
7. Counterstain with VG Staining Solution for 30 s, rinse rapidly with water, then immediately differentiate and dehydrate quickly with 95% ethanol.
8. Dehydrate in absolute ethanol, clear with xylene or dewaxing clearing agent, and mount with neutral balsam.
Staining Results:
Elastic fibers: Blue to bluish‑black
Collagen fibers: Red
Muscle fibers and erythrocytes: Yellow
Precautions:
1. The oxidation and bleaching steps may be omitted if only mature elastic fibers are to be demonstrated.
2. Acid Differentiation Solution mainly clarifies the background; slightly prolonged differentiation does not harm the staining.
3. Please use the reagents as soon as possible after opening to avoid affecting experimental results.
4. For your safety and health, wear a lab coat and disposable gloves during operation.
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