Mouse Anti-Human IgG (FITC) - Secondary antibody, specific to Human IgG

Cat. No.: Ab226748

GRADE & PURITY Validated, ExactAb™, 0.5mg/mL

Application

Flow Cytometry, IF/ICC

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG1

Conjugation

FITC

Descripción general

Mouse Anti-Human IgG Antibody (mAb), Secondary antibody, could be used for IF/ICC and Flow Cytometry.

Specifications

Product Name

Mouse Anti-Human IgG (FITC) - Secondary antibody, specific to Human IgG

Sinónimos

Mouse Anti-Human IgG Antibody | Mouse Anti Human IgG Antibody | Anti-Human IgG Antibody | Anti Human IgG Antibody | Human IgG Antibody | FLJ39988 antibodyFLJ40036 antibody | FLJ40253 antibody | FLJ40587 antibody | FLJ40789 antibody | FLJ40834 antibody | G

Especificaciones y pureza

ExactAb™, Validated, 0.5mg/mL

Especies hospedadoras

Mouse

Especificidad

Human IgG

Inmunógeno

Human IgG

Conjugación

FITC

Excitación (nm)

Blue Laser(488nm)

Emisión (nm)

517nm

Ex/Em(nm)

Ex:498nm;Em:517nm

Grado

ExactAb™, Validated

Tipo de cadena ligera

kappa

Método de purificación

Octylic acid-ammonium sulfate precipitation

Concentración

0.5mg/mL

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Protected from light,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imágenes

IF/ICC: Mouse Anti-Human IgG (FITC) (Ab226748)

Mouse Anti-Human IgG (FITC) (Ab226748) - IF/ICC
IF analysis of EpCAM (green) in HT-29 cells. The cells were fixed and permeabilized with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Citatuzumab (anti-EpCAM) (Ab209902) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Mouse Anti-Human IgG (FITC) (Ab226748) at a dilution of 1/500 for 1 hour at room temperature in the dark (green). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: Mouse Anti-Human IgG (FITC) (Ab226748)

Mouse Anti-Human IgG (FITC) (Ab226748) - Flow Cytometry
Two-color flow cytometric analysis of IgG expression on viable Human peripheral blood lymphocytes. Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD19 Antibody (APC) (Ab224677) and either Mouse IgG (FITC) (Ab180307; Left panel) or Mouse Anti-Human IgG (FITC) (Ab226748; Right panel) at 0.3μg/test.

Flow Cytometry: Mouse Anti-Human IgG (FITC) (Ab226748)

Mouse Anti-Human IgG (FITC) (Ab226748) - Flow Cytometry
Flow Cytometry analysis of HT-29 cells labelling EpCAM (red) with Citatuzumab (anti-EpCAM) (Ab209902). Mouse Anti-Human IgG (FITC) (Ab226748) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, Human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

• Aplicación: Dilution info
• IF/ICC:

  1:500 ;

• Flow Cytometry:

  1/50 - 1/1000