MRT-83 - 10mM in DMSO , CAS No.1263131-92-5

CAS: 1263131-92-5 Cat. No.: M654483 Fórmula: C₃₁H₃₀N₄O₅ Peso molecular: 538.59

GRADE & PURITY 10mM in DMSO

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Descripción general

MRT-83 is a potent antagonist of Smo , with an IC 50 in the nanomolar range. MRT-83 also blocks Hedgehog (Hh) signaling.

In Vitro

MRT-83 displays full antagonist properties with an IC 50 (~3 nM) for inhibiting ShhN (3 nM)-induced proliferation of rat GCPs. MRT-83 also blocks SAG (0.01 μM)-induced proliferation of GCPs (IC 50 ~6 nM). MRT-83 blocks BC binding to HEK-hSmo cells in a dose-dependent manner with an IC 50 of 4.6 nM. MRT-83 abrogates BC binding to cells expressing mouse Smo with an IC 50 of 14 nM, which is in good correlation with its IC 50 in the Shh-light2 and alkaline phosphatase assays. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Animals treated with ShhN in the presence of MRT-83 are as healthy as those of the other groups but up-regulation of Ptc transcription in the SVZ of these animals is no longer observed in agreement with a complete inhibition of ShhN-mediated effects (8.7±2.4 Ptc + cells/section, n=9) and is not different from vehicle-mediated effects. MRT-83 but not MRT-36 antagonizes the up-regulation of Ptc transcription induced by ShhN in vivo in the SVZ of the LV . MCE has not independently confirmed the accuracy of these methods. They are for reference only.

IC50& Target:Smo

Specifications

Especificaciones y pureza

10mM in DMSO

Mecanismos bioquímicos y fisiológicos

MRT-83 is a potent antagonist of Smo , with an IC 50 in the nanomolar range. MRT-83 also blocks Hedgehog (Hh) signaling.

Condiciones de almacenamiento de almacenamiento

Store at -80°C

Enviado en

Dry ice packs + Cold packs

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Nombres e identificadores

• Isómeros SMILES: CC1=C(C=C(C=C1)N=C(N)NC(=O)C2=CC(=C(C(=C2)OC)OC)OC)NC(=O)C3=CC=C(C=C3)C4=CC=CC=C4
• CAS alternativo: 1359944-60-7
• Peso molecular: 538.59

Objetivos asociados (humanos)

SMO Tclin Smoothened homolog (2 Activities)

Discover Target: SMO

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Preguntas frecuentes

How should this product be stored?

Store at ?80 °C. Ultra-low temperature storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 1263131-92-5, the molecular formula is C31H30N4O5, and the molecular weight is 538.59 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.