β-N-Acetylglucosaminidase, CAS No.9012-33-3

CAS: 9012-33-3 Cat. No.: N755074 Número EC: 232-730-2

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, 4000 U/ml; expressed in E.coli

Accession #

P49610

Expression system

E. coli

Bioactivity

4000 U/ml

Descripción general

  β-N-Acetylglucosaminidase, sometimes called hexosaminidase, is reported to liberate terminal β-linked N-acetylglucosamine and N-acetylgalactosamine from a variety of substrates. The activity of β-N-actylglucosaminidase may be determined with the chromogenic substrate p-nitrophenyl-N-acetyl-β-D-glucosaminide. β-N-actylglucosaminidase hydrolyzes the terminal nonreducing N-acetyl-D-hexosamine residues. This enzyme contains two predominant isozymes, Hex A, a heterodimer, and Hex B, a homodimer. N-acetylglucosamine, acetamide, N-2-acetamido-2-deoyglucosylamine, N-acetylnojirimycin, and N-acetyldeoxynojirmycin are known inhibitors.

Applications

β-N-Acetylglucosaminidase is an enzyme involved in the hydrolysis of terminal N-acetyl-D-hexosamine residues in N-acetyl-β-D-hexosaminides.

Component List

N755074	Component	50U	Storage
N755074A	β-N-Acetylglucosaminidase (4000 U/ml)	12.5μl	-20℃. Avoid freeze/ Thaw cycle.
N755074B	10× GlycoBuffer 1	500μl	-20℃. Avoid freeze/ Thaw cycle.

Reaction Conditions

1× GlycoBuffer 1

Incubate at 37°C

1× GlycoBuffer 1:

5 mM CaCl₂, 50 mM sodium acetate (pH 5.5 @ 25°C)

Product Notes

1. Reactions may be scaled-up linearly to accommodate larger reaction volumes.

2. The amount of exoglycosidase enzyme required varies when different substrates are used. Start with 1–2 μl for 1 μg of glycoprotein or 100 nM of oligosaccharide for one hour in a 10–25 μl reaction. If there is still undigested material, let the reaction go overnight.

3. This product cannot be heat inactivated.

Specifications

Product Name

β-N-Acetylglucosaminidase, CAS No.9012-33-3

Sinónimos

β-N-Acetyl-D-hexosaminide N-acetylhexosaminohydrolase | β-N-Acetylhexosaminidase | beta-N-acetylhexosaminidase | beta-hexosaminidase | hexosaminidase | N-acetyl-beta-glucosaminidase | β-GlcNAcase | strH

Grado

ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™

Especificaciones y pureza

Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, 4000 U/ml; expressed in E.coli

Mecanismos bioquímicos y fisiológicos

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.

Bioactividad

4000 U/ml

Número de adhesión

P49610

Peso molecular previsto

125 kDa

CAS

9012-33-3

Número de la Comisión de Enzimas

EC 3.2.1.52

Tipo de molécula

Enzyme

Almacenamiento y envío

Concentración

4000 U/ml; expressed in E.coli

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Definición de unidad

One unit is defined as the amount of enzyme required to cleave > 95% of the terminal, non-reducing β-N-Acetylglucosamine from 1 nmol GlcNAcβ1-4GlcNAcβ1-4GlcNAc-7-amino-4-methylcoumarin (AMC), in 1 hour at 37°C in a total reaction volume of 10 μl.