Nm4-64 neuron fluorescent probe

Cat. No.: N598338

Storage

Protected from light,Store at -20°C

Shipped In

Ice chest + Ice pads

Descripción general

Product introduction：

Nerve terminal probes are a series of cationic styryl fluorescent dyes used to track the synaptic activity of neuromuscular connections or synapses. Such dyes usually have lipophilic tails (two carbon chains) and highly hydrophilic heads with cations. The nerve terminal dye was named nervered or nervegreen followed by a carbon number, indicating the length of the lipophilic tail. Nm dyes have a similar structure, in addition, they have aldehyde fixed amines in the positively charged head. Cationic styryl dyes function by activity dependent staining of synaptic vesicles. When the dye is co incubated with cells or tissues, the aqueous part of the dye has no fluorescence, while the lipophilic tail of the dye inserts into the cell membrane and exhibits strong fluorescence. After nerve stimulation, the dye is encapsulated in the vesicles during endocytosis. Therefore, after washing the dye attached to the cell surface, the fluorescence signal intensity indicates the number of newly formed vesicles. On the contrary, during exocytosis, the dye is released from the vesicles together with neurotransmitters, resulting in the reduction of fluorescence signal. Therefore, the change in fluorescence intensity reflects the situation of endocytosis / exocytosis or synaptic activity. The rate of fluorescence increase during endocytosis - "binding rate" and the rate of fluorescence decrease during exocytosis - "dissociation rate" vary according to the dye type. In general, dyes with longer lipophilic tails and more double bonds have higher affinity for membranes and thus higher binding rates and lower dissociation rates. This product is equivalent to am4-64. Nm4-64 dye can be used for fixed cell staining.

Product parameters：

Appearance: Purple solid soluble in water ex/em:543/- nm, (emission in MeOH is too weak to measure) ex/em:510/750 nm (in membranes) molecular formula: c29h45n4cl3 molecular weight: 555.5

Scope of application：

Neurofluorescent dye

Specifications

Condiciones de almacenamiento de almacenamiento

Protected from light, Store at -20°C

Enviado en

Ice chest + Ice pads

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