Nuclear Fast Red Staining Solution (0.1%) - BioReagent, Biological Stain, for Microscopy, 0.1% in Deionized water, used for 生物染色 various applications , CAS No.6409-77-4

CAS: 6409-77-4 Cat. No.: N743376 Peso molecular: 357.28 Número EC: 229-088-0

GRADE & PURITY BioReagent, Biological Stain, for Microscopy, 0.1% in Deionized water

Synonyms

Nuclear Fast Red | Kernechtrot | Calcium Red

Storage

Protected from light,Room temperature

Shipped In

Normal

Descripción general

Nuclear Fast Red Staining Solution is a reagent for staining cell nuclei with nuclear fast red. It serves as a commonly used counterstain for tissue section staining, staining cell nuclei red after staining.Nuclear fast red, also known as Kernechtrot or Calcium Red, has the molecular formula C₁₄H₈NNaO₇S, molecular weight of 357.27 and CAS No. 6409-77-4. It appears as slightly reddish to dark brown powder, soluble in ethanol and water. As a widely applied counterstain in tissue section staining, nuclear fast red staining solution binds to cell nuclei and stains them red. It can also be used for the determination of calcium in serum. This staining solution is frequently used in combination with Prussian Blue Staining Solution.

Instructions for Use:

1. Sample Preparation

1.1 Paraffin sections: Deparaffinize in xylene, twice for 3–5 min each.

Dehydrate with absolute ethanol for 5 min, 90% ethanol for 2 min, 70% ethanol for 2 min, then rinse with PBS for 2 min.

1.2 Frozen sections: Rinse with PBS for 2 min.

1.3 Cultured cells: Fix with 4% paraformaldehyde for no less than 10 min, wash with PBS twice for 2 min each.

2. Nuclear Fast Red Staining

Counterstain with Nuclear Fast Red Staining Solution for 1–5 min. The staining duration can be adjusted according to staining effect and experimental requirements.

Staining Result:

Cell nuclei

Red

Precautions:

1. It is recommended to conduct a pilot test with 1–2 samples before formal experiments upon first use of this kit.

2. For personal safety, wear appropriate protective equipment such as lab coat and gloves before handling the reagent.

Specifications

Sinónimos

Nuclear Fast Red | Kernechtrot | Calcium Red

Especificaciones y pureza

BioReagent, Biological Stain, for Microscopy, 0.1% in Deionized water

Aplicación

生物染色

Estabilidad y almacenamiento

Store at room temperature long term (12 months). Store in the dark.

Condiciones de almacenamiento de almacenamiento

Protected from light, Room temperature

Enviado en

Normal

Grado

Biological Stain, BioReagent, for Microscopy

Nombres e identificadores

• Pubchem Sid: 528772206
• Pubchem Sid Url: https://pubchem.ncbi.nlm.nih.gov/substance/528772206
• Sonrisas canónicas: C1=CC=C2C(=C1)C(=O)C3=C(C2=O)C(=C(C(=C3N)O)S(=O)(=O)[O-])O.[Na+]
• IUPAC Name: sodium;4-amino-1,3-dihydroxy-9,10-dioxoanthracene-2-sulfonate
• InChIKey: IFSXZLJQEKGQAF-UHFFFAOYSA-M
• INCHI: 1S/C14H9NO7S.Na/c15-9-7-8(12(18)14(13(9)19)23(20,21)22)11(17)6-4-2-1-3-5(6)10(7)16;/h1-4,18-19H,15H2,(H,20,21,22);/q;+1/p-1
• Isómeros SMILES: C1=CC=C2C(=C1)C(=O)C3=C(C2=O)C(=C(C(=C3N)O)S(=O)(=O)[O-])O.[Na+]
• WGK Alemania: 1
• Peso molecular: 357.28
• Reaxy-Rn: 20093893
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=20093893&ln=

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Benzenoids
    • Clase Anthracenes
      • Subclass Anthraquinones

• Kingdom: Organic compounds
• Superclass: Benzenoids
• Clase: Anthracenes
• Subclass: Anthraquinones
• Intermediate Tree Nodes: Not available
• Direct Parent: Hydroxyanthraquinones
• Alternative Parents: Arylsulfonic acids and derivatives Aryl ketones Vinylogous amides Vinylogous acids Sulfonyls Organosulfonic acids Primary amines Organopnictogen compounds Organic sodium salts Organic oxides Hydrocarbon derivatives
• Molecular Framework: Aromatic homopolycyclic compounds
• Substituents: Hydroxyanthraquinone - Arylsulfonic acid or derivatives - Aryl ketone - Organic sulfonic acid or derivatives - Organosulfonic acid or derivatives - Organosulfonic acid - Sulfonyl - Vinylogous amide - Vinylogous acid - Ketone - Organic alkali metal salt - Organic sodium salt - Amine - Hydrocarbon derivative - Organic oxide - Primary amine - Organosulfur compound - Organooxygen compound - Organonitrogen compound - Organopnictogen compound - Organic oxygen compound - Organic nitrogen compound - Organic salt - Aromatic homopolycyclic compound
• Descripción: This compound belongs to the class of organic compounds known as hydroxyanthraquinones. These are compounds containing a hydroxyanthraquinone moiety, which consists of an anthracene bearing a quinone, and hydroxyl group.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Mecanismos de acción

Propiedades químicas y físicas

• Sensibilidad: light-sensitive
• Punto de fusión (°C): 300°C
• Peso molecular: 357.270 g/mol
• Hydrogen Bond Donor Count: 3
• Hydrogen Bond Acceptor Count: 8
• Rotatable Bond Count: 1
• Exact Mass: 356.992 Da
• Monoisotopic Mass: 356.992 Da
• Topological Polar Surface Area: 166.000 Ų
• Heavy Atom Count: 24
• Formal Charge: 0
• Complexity: 634.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 2

XLogP3

Referencias

1. Hongxia Xiu, Yajie Liu, Huihui Yang, Haibin Ren, Bowen Luo, Zhipeng Wang, Hong Shao, Fengzhong Wang, Jingjian Zhang, Yutang Wang.  (2022)  Identification of novel umami molecules via QSAR models and molecular docking.  Food & Function,  13  (14): (7529-7539).  [PMID:35765918] [10.1039/D2FO00544A]

2. Hui-Hui Xiao, Quan-Gui Gao, Ming-Xian Ho, Yan Zhang, Ka-Chun Wong, Yi Dai, Xin-Sheng Yao, Man-Sau Wong.  (2015)  An 8-O-4′ norlignan exerts oestrogen-like actions in osteoblastic cells via rapid nongenomic ER signaling pathway.  JOURNAL OF ETHNOPHARMACOLOGY,      [PMID:25978953] [10.1016/j.jep.2015.05.012]

3. Junling An, Hao Li, Wenjing Wen, Gaojian Liu, Zixuan Huang, Xinwei Zhao, Gaofeng Liang.  (2025)  Dual Inhibition of GPX4 and LCN2 via miR-214-3p Loaded Iron Oxide Nanoparticles for Enhancing Ferroptosis in Liver Cancer.  COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,      [PMID:] [10.1016/j.colsurfa.2025.137049]