Specific Activity ≥ 12 U/mg;Activity ≥ 1.25 U/ml for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Product Description
Specifictity
O-glycosidase cleaves only unsubstituted Gal-Beta(1-3)GalNAc-alpha disaccharides attached to the serine or threonine residues of glycoproteins or glycopeptides.
Substitutions such as sialic acid, galactose, fucose or N-acetylglucosamine must first be removed with the appropriate exoglycosidase prior to treatment with Endo-O-Glycosidase. At minimum, a sialadase such as Neuraminidase Au (alpha-2-3,6,8,9), part number E-S001, is almost always required to remove sialic acids
Source Recombinant Streptococcus pneumoniae in E.Coli
EC 3.2.1.97
CAS 9032-92-2
Specific Activity >12 U/mg
Activity >1.25 U/ml
Molecular weight 180,000 daltons
pH range 5-7, optimum 5.0
Purity:O-Glycosidase is tested for contaminating protease as follows; 10 μg of denatured BSA is incubated for 24 hours at 37°C with 2 μL of enzyme. SDS-PAGE analysis of the treated BSA shows no evidence of degradation.
Specific Activity Defined as the amount of enzyme required to produce 1 µmole of p-nitrophenol (pNP) in 1 minute at 37˚C, pH 5.0 from p-nitrophenyl- 2-acetamido-2-deoxy-3-O-(Beta-D-galactopyranosyl)-alpha- D-galactopyranoside.
Contents
60 µl aliquot of enzyme (75 mU) in 50 mM sodium phosphate, pH 7.5.
Included with 20 µL and 60 µL pack sizes:
5x Reaction Buffer 5.0 (250 mM sodium phosphate, pH 5.0)
Suggested usage
1. Add up to 100 µg of glycoprotein to a tube.
2. Add de-ionized water to a total of 13 µl.
3. Add 4 µl of 5x Reaction Buffer 5.0.
4. Add 1 µl of Neuraminidase Au (alpha 2-,3,6,8,9), part number E-S001.
5. Add 2 µl of O-Glycosidase.
6. Incubate for 1 hour at 37˚C.
Storage
Store enzyme at 4˚C.
Stability Stable at least 12 months when stored properly. Several days exposure to ambient temperature will not reduce activity. Active for at lease 5 days under reaction conditions.
| Peso molecular | ~180000 |
|---|
Comprehensive hazard, handling, storage, and regulatory compliance document.
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