Polyethylene glycol (PEG) is odorless, non-toxic, non-irritating, and has low immunogenicity. It exhibits good water solubility, low non-specific binding to proteins and cells, and excellent biocompatibility. As a synthetic polymer approved by the FDA, it can be used for human injection.
PEGNB is a photosensitive polymer material obtained by modifying polyethylene glycol (PEG) with o-nitrobenzyl alcohol (NB). The NB group can undergo photolysis under light irradiation at a wavelength of 365–405 nm, producing aldehyde groups that can react with amino groups. This enables the covalent conjugation of PEGNB with amino-containing substances (such as proteins, peptides, drugs, bioactive factors, etc.), as well as seamless integration and adhesion to tissues. Furthermore, when used in combination with photosensitive polymers containing double bonds, the photolysis products of NB can participate in free-radical polymerization to form hydrogels with excellent mechanical strength.
Product Specifications
| Component | Appearance | Specification | Remarks | PEGNB
| Pale yellow to yellow powder or granules
| 1 g/bottle, 5 g/bottle | Store in the dark
|
|
Suggestions for Use
1. PEGNB can be used in combination with amino-containing polymers such as polylysine, chitosan, carboxymethyl chitosan, gelatin, etc. Cross-linking occurs under light irradiation at 365–405 nm to form a gel.
2. PEGNB can be used in combination with methacryl-modified polymers such as CMCMA, HAMA, CSMA, ChMA, CMChMA. Polymerization is initiated by a photoinitiator under light irradiation to construct hydrogel materials with different physicochemical properties.
Preparation Method
1. When used with double bond-containing polymers and a photoinitiator, the recommended dosage of photoinitiator is as follows:
O775165 (MW 40 kDa): 63 mg (LAP/NAP) per 1 g PEGNB;
N1518263 (MW 80 kDa): 32 mg (LAP/NAP) per 1 g PEGNB.
2. Preparation of photoinitiator stock solution (if applicable):
Dissolve an appropriate amount of NAP or LAP to prepare a solution of the desired concentration. Store at 2-8 °C in the dark.
3. Weigh the required amount of PEGNB into a centrifuge tube. Add the required volume of initiator stock solution or prepared solution to the tube.
4. Stir the sample in the dark at room temperature or shake on an orbital shaker until completely dissolved. If the sample clumps in solution, crush it gently with a spatula first. If dissolution is slow, heat and stir at 50–60 °C to assist solubilization.
Precautions
1. Higher concentrations of PEGNB solution result in stronger tissue adhesion, but also higher light blocking and longer required irradiation time.
For 40 kDa PEGNB: recommended concentration ≤ 30 wt%.
For 80 kDa PEGNB: recommended concentration ≤ 50 wt%.
2. Higher molecular weight of PEGNB leads to better elasticity and toughness of hydrogels formed with double bond-containing photosensitive polymers.
Product Applications
3D bioprinting, drug sustained release, tissue engineering, regenerative medicine, protein immobilization, bone/cartilage repair, postoperative anti-adhesion, etc.
Sterilization Methods
1. Filter sterilization (recommended): Sterilize the solution using a 0.22 μm sterile syringe filter. This method is only suitable for low-viscosity solutions. For high-viscosity solutions, use pasteurization or autoclaving.
2. Pasteurization: Heat the solution to 80 °C and hold for 30 min, then quickly cool to room temperature in an ice-water bath. Repeat the cycle three times.
3. Autoclaving (moist heat sterilization): Sterilize the solution in an autoclave at 121 °C for 8 min. After manual venting, cool rapidly to room temperature in an ice-water bath. Note that high temperature may reduce gel performance; strictly follow the above procedure.
Note: Sterile solutions: store at 2–8 °C in the dark, use within 7 days. Non-sterile solutions: store at 2–8 °C in the dark, use within 48 hours.
Product Features
1. In situ, rapid formation of high-strength and high-toughness hydrogels: Can form high-strength, tough, bionic interface-bonded nanocomposite hydrogels with HAMA in seconds.
2. Tissue adhesion and integration: Aldehyde groups generated by photolysis of PEG-NB react with amino groups on the tissue surface at the application site, achieving covalent bonding between the gel and tissue.
Typical Applications
Based on the above advantages, PEGNB hydrogels have been used by researchers worldwide for 3D printing and drug sustained release, achieving a series of landmark results.
1. DLP printing: Fabrication of high-structural-complexity, micron-precision hydrogel scaffolds, blood vessels and other devices.
2. Medical applications:
“One-step” construction of lacrimal duct occlusion and ocular surface sustained-release drug delivery systems.
Compos. B. Eng. (IF = 13.1), 2023, 266, 111014
Product Advantages
1. Batch-to-batch stability: Standardized production process ensures high repeatability and consistency in composition, physicochemical properties and biological performance between different batches.
2. Ultra-high purity: Unique production process effectively removes small-molecule NB and process impurities, achieving an ultra-low impurity level and ensuring safety for biomedical applications.
3. Reliable safety: PEGNB is the first commercially available PEGNB product worldwide that has completed dual biocompatibility evaluation by NIFDC and a third-party institution, as well as third-party toxicology and metabolism studies. The product is safe, non-toxic, biodegradable, absorbable, distributable and excretable, accelerating the translation of downstream products from lab to clinic.
4. High stability: In accordance with the Guidance for Stability Study of Non-Invasive Implantable Medical Devices, real-time and accelerated stability tests confirm that PEGNB remains stable under labeled storage conditions.
ZJ26F0535266