GRADE & PURITYAnimal Free?Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material.Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)1.0 mg/mL
Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence.
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circum
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Imágenes
p53 Mouse mAb (Ab169235) - Western Blot All lanes: p53 Mouse Antibody (Ab169235) at 1/10000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 44 kDa Observed band size: 52 kDa Exposure time: 30.0 s
p53 Mouse mAb (Ab169235) - Flow Cytometry Flow Cytometry analysis of HEK293 cells stained with p53 Mouse mAb (Ab169235) (Red) at 2.5 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. Mouse IgG2b-kappa, anti-E8L (Ab210600) was used as an isotype control (Blue).
Aplicación
Aplicación
Dilution info
WB
1/1000 - 1/20000
Flow Cytometry
1/200 - 1/3
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Dilution Calculator
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Reconstitution Calculator
Reseñas
Reseñas de cliente
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