Papain

Cat. No.: P755552

GRADE & PURITY from Carica papaya

Descripción general

Papain is a cysteine protease of the papain family. It is a single chain protein. The molecular weight of papain is 23 kDa. Papain is composed of two structural domains and a cleft between them, which consists of the active site.

Application:

Papain has been used in the digestion of various brain cells.

Use Papain for the complete proteolytic cleavage of proteins, limited hydrolysis of native immunoglobulins, and tissue dissociation (together with collagenase, esterase, and trypsin). The enzyme solubilizes integral membrane proteins and produces glycopeptides from purified proteoglycans. The addition of cysteine (approximately 0.5% [w/v]) is essential for enzyme activity.

Specifications

Product Name

Papain

Especificaciones y pureza

from Carica papaya

Mecanismos bioquímicos y fisiológicos

Papain exhibits proteolytic activity against amide links, amino acid esters, peptides, proteins. It mainly cleaves peptide bonds containing amino acids such as lysine, arginine and residues succeeding phenylalanine. It can catalyze several reactions, such

Número de la Comisión de Enzimas

3.4.22.2(BRENDA, IUBMB)

Tipo de molécula

Small molecule

Almacenamiento y envío

Concentración

from Carica papaya

Condiciones de almacenamiento de almacenamiento

Room temperature

Enviado en

Normal

Estabilidad y almacenamiento

Store at 2 to 8 °C. (a decrease in activity of 20% may occur within 6 months)