Phospholipid Iron Hematoxylin (FeH) Staining Solution

Cat. No.: P1508444

GRADE & PURITY BioReagent, Biological Stain, for Microscopy

Descripción general

Phospholipids are lipids containing phosphate groups and belong to complex lipids. As key components of biological membranes, phospholipids are classified into two categories: glycerophospholipids and sphingophospholipids. Phospholipids are amphipathic molecules, with a hydrophilic head containing nitrogen or phosphorus at one end and a hydrophobic (lipophilic) long hydrocarbon chain at the other end.

Phospholipid Iron Hematoxylin (FeH) Staining Solution was invented by Elleder. Its principle is that phospholipids can be visualized using ferric hematoxylin. Compared with the DAH method, this method is simpler, faster and more sensitive. However, it has drawbacks: tissues need to be defatted with acetone to achieve optimal staining results, and sometimes both cell nuclei and lipids are stained simultaneously, showing a black-blue color. Note: This reagent is for research use only and not intended for clinical diagnosis or other purposes.

P1508444	Component	3×100 mL	Storage
P1508444A	FeH Control Solution	10 mL	RT. Store in the dark.
P1508444B	FeH Fixative Solution	100 mL	RT.
P1508444C	FeH Hematoxylin Staining Solution	25 mL	RT. Store in the dark.
P1508444D	FeH Buffer	75 mL	RT.
P1508444E	FeH Differentiating Solution	100 mL	RT.

Reagents C and D can be mixed to prepare the FeH Stain.

Materials to Be Prepared by User

Chloroform, acetone, distilled water, xylene or environment-friendly deparaffinization and clearing solution, DPX mounting medium

Operating Procedures (For Reference Only)

1. Take two consecutive sections. Immerse one section in FeH Control Solution at room temperature for 1 hour as a control; soak the other section in anhydrous acetone at 4℃ for 15 minutes.

2. Fix the sections in FeH Fixative Solution for 30 minutes, then rinse with distilled water.

3. Prepare the FeH Stain by mixing FeH Hematoxylin Stain and FeH Buffer at a ratio of 1:3.

4. Stain the sections in the FeH Stain for 7–10 minutes, followed by rinsing with distilled water.

5. Dilute the FeH Differentiating Solution with an equal volume of distilled water, then immerse the sections in the diluted solution several times.

6. Rinse the sections with tap water.

7. Dehydrate the sections with acetone, clear them with xylene or deparaffinization and clearing solution, and mount the sections with DPX mounting medium.

Staining Results

Phospholipids: Blue

Cell Nuclei: Blue

Precautions

1. Phospholipids are prone to dissolution. Therefore, tissues should be fixed immediately after collection; otherwise, it will be difficult to achieve satisfactory staining.

2. This stain solution yields optimal results with frozen sections.

3. Stained specimens must be stored away from light to prevent fading.

4. For your safety and health, wear a lab coat and disposable gloves during operation.

5. Use the reagent as soon as possible after opening to avoid affecting the results of subsequent experiments.

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Protected from light,Room temperature

Enviado en

Normal

Contents & Storage

P1508444	Component	3×100 mL	Storage
P1508444A	FeH Control Solution	10 mL	RT. Store in the dark.
P1508444B	FeH Fixative Solution	100 mL	RT.
P1508444C	FeH Hematoxylin Staining Solution	25 mL	RT. Store in the dark.
P1508444D	FeH Buffer	75 mL	RT.
P1508444E	FeH Differentiating Solution	100 mL	RT.