GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)See COA
Carrier Free,ExactAb™,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE),See COA Azide Free,Carrier Free,ExactAb™,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Mouse anti Phosphotyrosine Antibody, Monoclonal (4G10), could be used for Flow, ELISA, WB and so on. Application: Flow: Use at an assay dependent concentration. ELISA: Use at an assay dependent concentration. WB: Use at an assay dependent concentration.
Specifications
Product Name
Phosphotyrosine Mouse mAb - Primary antibody, specific to Phosphotyrosine, Mouse IgG2b
Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.
Imágenes
Phosphotyrosine Mouse mAb (Ab121716) - Flow Cytometry Flow Cytometry analysis of unstimulated (left) or 1 mM sodium vanadate (S105283) stimulated (right) NIH3T3 mouse embryonic fibroblast cells labelling Phosphotyrosine Mouse mAb (red) with Phosphotyrosine Mouse mAb (Ab121716) at 0.2 μg/mL for 1 hour at 4°C, and detected with Goat anti-Mouse IgG (PE) (Ab179006). Blue - Mouse IgG Isotype Control Antibody (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
Phosphotyrosine Mouse mAb (Ab121716) - Flow Cytometry Flow Cytometry analysis of Jurkat cells was cultured in serum-free media overnight and treated with 5 mM H2O2 for 15 minutes labelling Phosphotyrosine Mouse mAb (red) with Phosphotyrosine Mouse mAb (Ab121716) conjugated with biotin at 0.2 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.
Aplicación
Aplicación
Dilution info
WB
Use at an assay dependent concentration.
Flow Cytometry
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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