Plant Callose Staining Solution (Aniline Blue Visible-Light Method) - BioReagent,for microscopy,Biological Stain

Cat. No.: P1511469

GRADE & PURITY BioReagent, Biological Stain, for Microscopy

Synonyms

Aniline Blue Callose Stain (Visible Light) | Water-Soluble Aniline Blue Callose Staining Solution | Callose Visualization Solution (Aniline Blue, Visible Light) | Aniline Blue Stain for Plant Callose (Non-fluorescent) | Plant Callose Detection Solution (A

Storage

Protected from light,Room temperature

Shipped In

Normal

Descripción general

  Callose is a type of non-cellulosic polysaccharide composed of glucose residues linked in a manner distinct from cellulose. It plays a crucial regulatory role in key biological processes such as plant sieve tube metabolism and gametophyte development. The processes of its synthesis and degradation directly impact normal plant growth and metabolism, making it a significant subject in botanical research.

  Plant Callose Staining Solution (Aniline Blue Visible-Light Method) utilizes the specific staining property of aniline blue for callose, clearly staining it blue. This facilitates direct observation and analysis under a visible light microscope. This product is intended for research use only and must not be used for clinical diagnosis or other non-research purposes.

Materials to Be Prepared by User

• AAF Fixative Solution or Carnoy's Fixative Solution, 50% Ethanol, Absolute Ethanol, 1× PBS
• Fresh plant tissue

Procedure (For Reference Only)

1. Cut fresh plant tissue into slices approximately 2 mm thick. For plant leaves, it is recommended to cut them into pieces about 1 cm * 2 cm. Tender plant leaves can be directly immersed in AAF Fixative Solution or Carnoy's Fixative Solution for 24 hours.

2. Rinse the tissue twice with absolute ethanol, 1 minute each time. Then transfer and store the tissue in at least 10 times its volume of 100% ethanol.

3. Before staining, retrieve the tissue and immerse it in 50% ethanol for equilibration for 30 minutes. Remove and allow to drain slightly.

4. Subsequently, immerse the tissue in 1× PBS for equilibration for 30 minutes. Remove and allow to drain slightly.

5. Apply the Callose Staining Solution dropwise onto the tissue section or immerse the section in the staining solution. Stain at room temperature, protected from light, for 4~24 hours.

6. Rinse the tissue section with distilled water. Carefully transfer the stained tissue onto a glass slide. Add a small amount of glycerol-gelatin mounting medium before coverslipping and observation.

Staining Results

  Callose appears blue.

Notes

1. When conditions permit, it is recommended to subject the plant tissue immersed in fixative to negative pressure/vacuum treatment for 20 minutes to aid fixative penetration.

2. If the experiment is not to be performed immediately or a large number of samples need to be processed simultaneously, samples stored in 100% ethanol can be kept at 2-8°C for at least one week.

3. Photograph the stained samples as soon as possible after staining to preserve the results.

4. Use the reagents as soon as possible after opening to avoid affecting subsequent experimental results.

5. For your safety and health, please wear a lab coat and disposable gloves during operation.

Specifications

Sinónimos

Aniline Blue Callose Stain (Visible Light) | Water-Soluble Aniline Blue Callose Staining Solution | Callose Visualization Solution (Aniline Blue, Visible Light) | Aniline Blue Stain for Plant Callose (Non-fluorescent) | Plant Callose Detection Solution (A

Especificaciones y pureza

BioReagent, for microscopy, Biological Stain

Estabilidad y almacenamiento

Store at room temperature long term (12 months). Store in the dark.

Condiciones de almacenamiento de almacenamiento

Protected from light, Room temperature

Enviado en

Normal

Grado

Biological Stain, BioReagent, for Microscopy

Propiedades químicas y físicas

• Sensibilidad: Light-sensitive