Plant Tissue Staining Solution (Delafield Hematoxylin‑Safranin Method)

Cat. No.: P1511516

GRADE & PURITY BioReagent, Biological Stain, for Microscopy

Descripción general

  Plant tissue staining methods are diverse, and their operational procedures are similar to those used for animal tissue staining. Delafield’s hematoxylin is an excellent stain suitable for non-lignified tissues. When used in combination with safranin as a counterstain, it can achieve ideal staining results for various plant disease tissues.

  The Plant Tissue Staining Solution (Delafield’s Hematoxylin-Safranin Method) mainly consists of Delafield’s Hematoxylin Staining Solution, Safranin Staining Solution, and other components. It stains lignified tissues red and structures such as fungal hyphae blue. This product is intended for research use only and is not suitable for clinical diagnosis or other non-research purposes.

Materials to Be Prepared by User

  10% neutral formalin fixative, distilled water, graded ethanol series

Procedure (For Reference Only)

1. Sample Processing: Fix the specimen and prepare paraffin sections.

2. Section Mounting: Cut the material into thin sections. Mount the sections onto clean glass slides and heat them to flatten. First, apply an adhesive to the slide, then place the section onto the adhesive solution. Position the slide on a warming plate to flatten the section, ensuring no wrinkles appear.

3. Dewaxing: Xylene → Xylene + absolute ethanol (1:1) → 100% ethanol → 95% ethanol → 85% ethanol → 70% ethanol → 50% ethanol → 30% ethanol → water. Incubate for 5–10 minutes at each step.

4. Stain in Delafield’s Hematoxylin Staining Solution for 30 minutes, then rinse with water for 1 minute.

5. Differentiate with Acidic Differentiation Solution and rinse with tap water.

6. Transfer to 30% ethanol for 1 minute, then to 50% ethanol for 1 minute.

7. Stain with Safranin Staining Solution for 1–6 hours. For special tissues, staining can be extended up to 12 hours.

8. Destaining: 50% ethanol → 70% ethanol → 80% ethanol → 90% ethanol → 95% ethanol. Incubate for 1–2 minutes at each step.

9. Clearing: 50% xylene + 50% ethanol for 2 minutes → xylene for 2 minutes → xylene for 10 minutes.

10. Mount with resin and examine under a microscope promptly.

Staining Results

Target Structure	Color
Lignified, suberized, cutinized tissues	Red
Fungal hyphae	Blue

Notes

1. Destaining in 50% ethanol after safranin staining requires practice. Insufficient destaining may affect subsequent staining, while excessive destaining can result in overly faint red coloration.

2. Xylene can be replaced with a dewaxing/clearing solution, which is an environmentally friendly alternative.

3. Staining times are not absolute and often vary depending on the type of material and section thickness.

4. For your safety and health, please wear a lab coat and disposable gloves during operation.

5. Use the reagents as soon as possible after opening to avoid affecting subsequent experimental results.

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Room temperature

Enviado en

Normal

Estabilidad y almacenamiento

Store at room temperature long term (12 months).

Contents & Storage

P1511516	Component	3×50 mL	Storage
P1511516A	Delafield’s Hematoxylin Staining Solution	50 mL	RT.
P1511516B	Acidic Differentiation Solution	100 mL	RT.
P1511516C	Safranin Staining Solution	50 mL	RT.