PNGase F from Elizabethkingia meningoseptica, CAS No.83534-39-8

CAS: 83534-39-8 Cat. No.: P755140 Número EC: 964-131-3

GRADE & PURITY Recombinant, ready-to-use solution, expressed in E. coli

Descripción general

Recombinant PNGase F has been purified by affinity chromatography and dialyzed into a 50% glycerol solution with 10 mM potassium phoosphate pH 7.5 to produce a stable product. The product contains low levels of buffer salts. This highly purified material can be used for preparative deglycosylation or for analytical applications in gel, in solution, or on blot membranes. The enzyme can be removed from preparative operations by utilizing its C-terminal 6x histidine fusion tag. PNGase F from Elizabethkingia meningoseptica has been used in deglycosylation assay in human plasma samples and in deglycosylation of chondroitin sulfate proteoglycan.

Used to deglycosylate protein.

Specifications

Product Name

PNGase F from Elizabethkingia meningoseptica, CAS No.83534-39-8

Grado

Recombinant

Especificaciones y pureza

Recombinant, ready-to-use solution, expressed in E. coli

Mecanismos bioquímicos y fisiológicos

PNGase F from Elizabethkingia meningoseptica has glycan-binding catalytic domain and a bowl-like domain at the N-terminus. It cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl

CAS

83534-39-8

Número de la Comisión de Enzimas

3.5.1.52(BRENDA, IUBMB)

Tipo de molécula

Peptide

Almacenamiento y envío

Concentración

ready-to-use solution, expressed in E. coli

Condiciones de almacenamiento de almacenamiento

Store at -20°C

Enviado en

Ice chest + Ice pads

Referencias

1. Donghua Su, Xiaolong Zhang, Tianwu An, Xi Wang, Yaqiu Lin, Haibo Wang, Peiqiang Yu, Haitao Shi.  (2025)  Astral-DIA proteomics and 4D label-free N-glycoproteomics of milk fat globule membrane proteins reveal distinct profiles between yak colostrum and mature milk.  Food Chemistry-X,      [PMID:41378274] [10.1016/j.fochx.2025.103302]