In a narrow sense, connective tissue contains three types of fibers: collagen fibers, reticular fibers, and elastic fibers. Among them, collagen fibers are the most widely distributed and abundant fiber type. Masson's Trichrome Staining, also known as Masson staining, is the most classic method for connective tissue staining and an authoritative and standard technique for collagen fiber staining. Trichrome staining generally stains nuclei and selectively demonstrates collagen fibers and muscle fibers. The staining principle is related to the molecular size of anionic dyes and tissue permeability: Molecular size is reflected by molecular weight. Small molecules easily penetrate dense tissues with low permeability, whereas large molecules can only enter loose tissues with high permeability.
Ponceau Acid Fuchsin Staining Solution is one of the active components in Masson's Trichrome Staining, mainly used for staining muscle fibers, which appear red after staining. It is composed of Ponceau 2R, acid fuchsin, etc. This reagent is for research use only. Not for clinical diagnosis or other purposes.
Operating Procedures (For Reference Only):
1. Deparaffinize sections routinely to water, then rinse in distilled water for 1 min.
2. Stain in Ponceau Acid Fuchsin Staining Solution for 10 min, then rinse briefly with distilled water.
3. Perform downstream procedures as required. Rapidly dehydrate in 95% ethanol, then dehydrate in absolute ethanol 3 times, 5–10 s each. Clear in xylene 3 times, 1–2 min each. Mount with neutral balsam.
Staining Results:
Cytoplasm, muscle, red blood cells: Red
Precautions:
1. Section deparaffinization should be as thorough as possible.
2. Fixation plays an important role. Different fixatives may prolong or shorten the staining time.
3. Store tightly sealed; otherwise, volatilization of active components will reduce staining efficiency.
4. For your safety and health, wear laboratory coat and disposable gloves during operation.
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