Proline (Pro) is a cyclic α-imino acid. It is neutral with an isoelectric point of 6.30, and has a higher solubility in water than any other amino acid—approximately 162 g can be dissolved in 100 g of water at 25℃. It is hygroscopic and difficult to crystallize, with a sweet taste. When heated together with ninhydrin solution, it forms a yellow compound. Once incorporated into the peptide chain, proline can undergo hydroxylation to form 4-hydroxyproline, which is an important component of animal collagen. Hydroxyproline also exists in various plant proteins and is particularly associated with cell wall formation. Under normal conditions, the proline content is relatively low, but it often accumulates significantly under stress conditions (drought, heat, cold, freezing, salinization, etc.). The accumulation index is related to the stress resistance of plants. Proline has a wide range of applications in clinical practice, biomaterials, industry, and other fields.
This Proline (PRO) Extraction Reagent is mainly used for extracting proline from plant. 500 ml of the reagent can be used for approximately 100 sample extractions. Note: This reagent is intended for research purposes only and not for clinical diagnosis or any other applications.
Materials to Be Prepared by the User
1. Distilled water
2. Mortar or homogenizer, centrifuge tubes or test tubes, filter paper or gauze, water bath
Operating Procedures (For Reference Only)
1. Take fresh plant tissues, rinse them thoroughly, dry them, cut them into small pieces, and weigh 0.5 g of the samples quickly. Add 5 ml of PRO Extraction Reagent, homogenize or grind the mixture, heat it in a boiling water bath for 10 min (shake frequently during heating), and mix well.
2. Filter the mixture with filter paper or gauze. The filtrate is the proline extract, which can be stored at 4℃ for later use.
Precautions
1. The Proline (PRO) Extraction Reagent should be stored in a sealed container to avoid volatilization of active ingredients.
2. Please use the reagent as soon as possible after opening to prevent affecting the subsequent experimental results.
3. For your safety and health, wear a lab coat and disposable gloves during operation.
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