Pronase, from Streptomyces griseus, CAS No.9036-06-0

CAS: 9036-06-0 Cat. No.: P304928 Número EC: 232-909-5

GRADE & PURITY ~7000 units/g protein

Descripción general

Pronase is a mixture of several nonspecific endo- and exoproteases that digest proteins down to single amino acids.

Application:

Use Pronase to completely hydrolyze proteins in research applications. Pronase is used for the degradation of proteins during the isolation of DNA and RNA, such as in the extraction of phage DNA or the isolation of plasmid DNA. It is not necessary to let pronase self-digest prior to use. It is also used in histochemistry and cell culture for tissue dissociation in conjunction with collagenase and trypsin，and for the production of glycopeptides from purified glycoproteins. It has been used for the dechorionation of Zebrafish embryos.

Specifications

Product Name

Pronase, from Streptomyces griseus, CAS No.9036-06-0

Especificaciones y pureza

~7000 units/g protein

Mecanismos bioquímicos y fisiológicos

Pronase has wide-ranging specificity. Therefore, it is commonly used when extensive or complete degradation of proteins is required. Its applications include protein analysis in cell organelles, removal of proteins during DNA and RNA isolation, and the pr

CAS

9036-06-0

Número de la Comisión de Enzimas

3.4.24.4

Tipo de molécula

Enzyme

Almacenamiento y envío

Concentración

~7000 units/g protein

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C

Enviado en

Wet ice

Definición de unidad

The amount of tyrosine per minute. For units designated as“U,” the amount of tyrosine was 1 μmol/min, and equal amounts of casein were extracted from casein at a temperature of 40 ° C and a pH of 7.5

Referencias

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