Aladdin's Protein A/G IgG Binding Buffer (1×) is a ready-to-use, phosphate-based buffer, specifically formulated for high-yield, nondenaturing antibody purification using Protein A, Protein G, or Protein A/G affinity supports. This carefully optimized solution ensures maximum antibody binding capacity while maintaining the structural integrity and biological activity of purified immunoglobulins, providing reliable performance for both research and production applications.
Key Features
Universal Compatibility: A single buffer compatible with Protein A, Protein G, and Protein A/G affinity media, providing a flexible and convenient purification solution.
Ready-to-Use Convenience: Pre-diluted 1× solution requires no additional preparation, saving time and ensuring consistent results
Preserves Antibody Integrity: The gentle, nondenaturing binding conditions help maintain the native structure and biological activity of purified antibodies.
Enhanced Binding Efficiency: Specially formulated phosphate system promotes high-specificity antibody binding to affinity matrices
Rigorous Quality Control: Undergoes strict filtration and quality testing to ensure exceptional batch-to-batch consistency and reliability.
Applications
This product is widely used in various antibody purification scenarios:
Purification of IgG and its subtypes from diverse sources such as serum, ascites fluid, or cell culture supernatants from multiple species including mouse, rabbit, and human.
Compatible with Protein A, Protein G, or Protein A/G agarose/magnetic beads for manual or automated purification protocol.
Recommended Protocol
1. Equilibrate affinity support with Protein A/G IgG Binding Buffer (1×).
2. Apply sample in binding buffer to the prepared resin.
3. Perform standard wash steps to remove unbound contaminants.
4. Elute purified IgG using Aladdin's IgG Elution Buffer (available in both low-pH and gentle high-salt formulations).
5. For optimal results, pair with Aladdin's Protein A/G Agarose for maximum binding capacity.
Storage & Stability
Store at 2-8°C; stable for 12 months from manufacture date.
Use aseptic techniques for extended storage of opened containers.
