Protein Sample Loading Buffer (Denaturing, non-Reducing, 5×) - BioReagent, suitable for electrophoresis, for PAGE, for western blot, 5×

Cat. No.: P1492036

GRADE & PURITY BioReagent, Suitable for electrophoresis, for PAGE, for Western blot, 5×

Synonyms

SDS-PAGE Sample Loading Buffer

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Application

Electrophoresis, PAGE, SDS-PAGE, WB

Descripción general

  This product is an improved, 5× concentrated protein loading buffer containing Bromophenol Blue as a tracking dye. It includes denaturing agents but excludes reducing agents such as DTT or β-mercaptoethanol. It is suitable for routine denaturing non-reducing polyacrylamide gel electrophoresis (SDS-PAGE) of protein samples.
Protocol
1.Add 1 volume of loading buffer to 4 volumes of protein sample (1:4 ratio) and mix thoroughly.
2.Heat the mixture in a boiling water bath for 3–5 minutes.
3.After cooling, centrifuge at high speed for 5 minutes. Use the supernatant for electrophoresis.
3.1 Note: If no insoluble debris is observed after adding the loading buffer, brief centrifugation is sufficient to collect the mixture.
3.2 If condensation forms on the tube lid after heating, gently flick the tube to mix the condensation with the sample before centrifugation to ensure uniform loading concentration.
Precautions
1.Wear a lab coat and disposable gloves for safety.
2.For research use only.

Specifications

Sinónimos

SDS-PAGE Sample Loading Buffer

Especificaciones y pureza

BioReagent, suitable for electrophoresis, for PAGE, for western blot, 5×

Estabilidad y almacenamiento

Store at -20℃ long term (12 months).

Condiciones de almacenamiento de almacenamiento

Store at -20°C

Enviado en

Ice chest + Ice pads

Este producto requiere envío en cadena de frío. Los servicios terrestres y otros servicios económicos no están disponibles.

Grado

BioReagent, for PAGE, for Western blot, Suitable for electrophoresis