GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.
BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Pyruvate carboxylase (PC, EC 6.4.1.1) is widely present in the mitochondria of animals, molds, and yeasts. It catalyzes the conversion of pyruvate, ATP, CO₂, and water to oxaloacetate, ADP, and inorganic phosphate (Pi). As the first rate-limiting enzyme of gluconeogenesis, it plays a crucial role in maintaining the dynamic balance of blood glucose.
Assay Principle: PC catalyzes the formation of oxaloacetate, ADP, and Pi from pyruvate, ATP, CO₂, and water. Malate dehydrogenase further catalyzes the conversion of oxaloacetate and NADH to malate and NAD⁺. The activity of PC is reflected by measuring the oxidation rate of NADH at 340 nm.
Almacenamiento y envío
Condiciones de almacenamiento de almacenamiento
Store at -20°C
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at -20℃ for 6 months.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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