GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.5mg/mL
ExactAb™, Validated, Recombinant, 0.5mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Recombinant CD19 Antibody, clone P08D14, could be used for Flow Cytometry.
Specifications
Product Name
Recombinant CD19 Antibody (Biotin) - Primary antibody, specific to Human CD19
Especificaciones y pureza
ExactAb™, Validated, Recombinant, 0.5mg/mL
Mecanismos bioquímicos y fisiológicos
Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes.
Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens.
Activates signaling pathways that lead to th
Especies hospedadoras
Mouse
Especificidad
Human CD19
Inmunógeno
Human Chronic Lymphocytic Leukemia (CLL) Cells.
Control positivo
Flow Cytometry: Human peripheral blood lymphocytes
Conjugación
Biotin
Grado
ExactAb™, Recombinant, Validated
Tipo de cadena ligera
kappa
Método de purificación
Protein A purified
Concentración
0.5mg/mL
Almacenamiento y envío
Condiciones de almacenamiento de almacenamiento
Store at 2-8°C,Protected from light,Do not freeze
Enviado en
Wet ice,Do not freeze
Estabilidad y almacenamiento
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Imágenes
Recombinant CD19 Antibody (Biotin) (Ab224660) - Flow Cytometry Flow cytometry analysis of CD19 expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Human peripheral blood cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD19 Antibody (Biotin) (Ab224660) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Mouse IgG (Biotin) (Ab180306).
Recombinant CD19 Antibody (Biotin) (Ab224660) - Flow Cytometry Flow cytometry analysis of CD19 expression by Raji cells (red) compared to an isotype control (blue). Cells were stained with Recombinant CD19 Antibody (Biotin) (Ab224660) at 0.3μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.
Recombinant CD19 Antibody (Biotin) (Ab224660) - Flow Cytometry Flow cytometry analysis of CD19 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Human peripheral blood cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD19 Antibody (Biotin) (Ab224660) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.
Aplicación
Aplicación
Dilution info
Flow Cytometry
≤1.0μg/ test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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