GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.5 μL/test
B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes.
Functions as a store-operated calcium (SOC) channel component promoting
Especies hospedadoras
Mouse
Especificidad
Human CD20
Inmunógeno
Human 6.16c1.3 B cell line
Control positivo
Flow Cytometry: Human peripheral blood lymphocytes
Conjugación
AF647
Excitación (nm)
Red Laser(633nm)
Emisión (nm)
668nm
Ex/Em(nm)
Ex:650nm;Em:668nm
Grado
ExactAb™, Recombinant, Validated
Tipo de cadena ligera
kappa
Método de purificación
Protein A purified
Concentración
5 μL/test
Almacenamiento y envío
Condiciones de almacenamiento de almacenamiento
Store at 2-8°C,Protected from light,Do not freeze
Enviado en
Wet ice,Do not freeze
Estabilidad y almacenamiento
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Imágenes
Recombinant CD20 Antibody (AF647) (Ab223962) - Flow Cytometry Flow cytometry analysis of CD20 expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD20 Antibody (AF647) (Ab223962) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (AF647) (Ab190077).
Recombinant CD20 Antibody (AF647) (Ab223962) - Flow Cytometry Flow cytometry analysis of CD20 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD20 Antibody (AF647) (Ab223962) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF647) (Ab190077). Black - Unlabelled control, cells without incubation with primary antibody.
Aplicación
Aplicación
Dilution info
Flow Cytometry
5μl/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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