GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.5mg/mL
Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
Especies hospedadoras
Rat
Especificidad
Mouse CD25
Inmunógeno
IL-2-dependent cytolytic mouse T-cell clone B6.1
Control positivo
Flow Cytometry: C57BL/6 mouse splenocytes
Conjugación
AF594
Excitación (nm)
Yellow-Green Laser(594nm)
Emisión (nm)
618nm
Ex/Em(nm)
Ex:590nm;Em:618nm
Grado
ExactAb™, Recombinant, Validated
Tipo de cadena ligera
lambda
Método de purificación
Protein A purified
Concentración
0.5mg/mL
Almacenamiento y envío
Condiciones de almacenamiento de almacenamiento
Store at 2-8°C,Protected from light,Do not freeze
Enviado en
Wet ice,Do not freeze
Estabilidad y almacenamiento
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Con A-stimulated (3 days) C57BL/6 mouse splenocytes cells were stained with Recombinant CD25 Antibody (AF594) (Ab230868) (red) compared to an isotype control (blue). Blue - Rat IgG (AF594) (Ab190241). Black - Unlabelled control, cells without incubation with primary antibody. Untreated C57BL/6 mouse splenocytes were used as control (left panel).
Aplicación
Aplicación
Dilution info
Flow Cytometry
≤1.0μg/ test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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