Recombinant CD45.2 Antibody (Biotin) - Primary antibody, specific to Mouse CD45

Cat. No.: Ab232045

GRADE & PURITY Recombinant, Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Mouse

Reactive species

Mouse

Isotype

Mouse IgG2a

Clone Number

P12F26

Conjugation

Biotin

Descripción general

Recombinant CD45.2 Antibody, clone P12F26, could be used for Flow Cytometry.

Specifications

Product Name

Recombinant CD45.2 Antibody (Biotin) - Primary antibody, specific to Mouse CD45

Sinónimos

B220 antibody | CD 45 antibody | CD45 antibody | CD45 antigen antibody | CD45R antibody | GP180 antibody | L-CA antibody | LCA antibody | Leukocyte common antigen antibody | loc antibody | Ly-5 antibody | LY5 antibody | Ly5, homolog of antibody | Lyt-4 an

Especificaciones y pureza

Recombinant, ExactAb™, Validated, 0.5 mg/mL

Mecanismos bioquímicos y fisiológicos

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affec

Especies hospedadoras

Mouse

Especificidad

Mouse CD45

Inmunógeno

B10.S mouse thymocytes and splenocytes

Control positivo

Flow Cytometry: C57BL/6 mouse splenocytes

Conjugación

Biotin

Grado

ExactAb™, Recombinant, Validated

Tipo de cadena ligera

kappa

Método de purificación

Protein A purified

Concentración

0.5 mg/mL

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Protected from light,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imágenes

Flow Cytometry: Recombinant CD45.2 Antibody (Biotin) (Ab232045)

Recombinant CD45.2 Antibody (Biotin) (Ab232045) - Flow Cytometry 

Flow cytometry analysis of CD45.2 expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD45.2 Antibody (Biotin) (Ab232045) at 0.3μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Mouse IgG (Biotin) (Ab180306).

Flow Cytometry: Recombinant CD45.2 Antibody (Biotin) (Ab232045)

Recombinant CD45.2 Antibody (Biotin) (Ab232045) - Flow Cytometry 

Flow cytometry analysis of CD45.2 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD45.2 Antibody (Biotin) (Ab232045) at 0.3μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

• Aplicación: Dilution info
• Flow Cytometry:

  ≤1.0μg/ test

  For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.;