GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.5 μL/test
ExactAb™, Validated, Recombinant, 5 μL/test ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Recombinant CD8α Antibody, clone P20A30, could be used for Flow Cytometry.
Specifications
Product Name
Recombinant CD8α Antibody (AF555) - Primary antibody, specific to Human CD8 alpha chain
Especificaciones y pureza
ExactAb™, Validated, Recombinant, 5 μL/test
Mecanismos bioquímicos y fisiológicos
Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide com
Especies hospedadoras
Mouse
Especificidad
Human CD8 alpha chain
Inmunógeno
Human CD8a
Control positivo
Flow Cytometry: Human peripheral blood lymphocytes
Conjugación
AF555
Excitación (nm)
Green Laser(532nm)
Emisión (nm)
568nm
Ex/Em(nm)
Ex:553nm;Em:568nm
Grado
ExactAb™, Recombinant, Validated
Tipo de cadena ligera
kappa
Método de purificación
Protein A purified
Concentración
5 μL/test
Almacenamiento y envío
Condiciones de almacenamiento de almacenamiento
Store at 2-8°C,Protected from light,Do not freeze
Enviado en
Wet ice,Do not freeze
Estabilidad y almacenamiento
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Imágenes
Recombinant CD8α Antibody (AF555) (Ab223591) - Flow Cytometry Flow cytometry analysis of CD8α expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Human peripheral blood cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD8α Antibody (AF555) (Ab223591) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (AF555) (Ab190246).
Recombinant CD8α Antibody (AF555) (Ab223591) - Flow Cytometry Flow cytometry analysis of CD8α expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Human peripheral blood cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD8α Antibody (AF555) (Ab223591) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF555) (Ab190246). Black - Unlabelled control, cells without incubation with primary antibody.
Aplicación
Aplicación
Dilution info
Flow Cytometry
5μl/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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