GRADE & PURITYRUO?Research Use Only — for laboratory research, not diagnostic or therapeutic use. Use in R&D; do not use in clinical diagnosis or patient treatment.Recombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.Suitable for Flow Cytometry?Flow-cytometry grade — validated antibodies/reagents for FACS analysis. Use to stain and quantify cell populations by flow cytometry.0.5 mg/mL
Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide com
Especies hospedadoras
Rat
Especificidad
Mouse CD8α
Inmunógeno
Mouse Spleen Cells or Thymocyte Membranes
Control positivo
Flow Cytometry: C57BL/6 mouse splenocytes
Conjugación
APC
Excitación (nm)
Red Laser(633nm)
Emisión (nm)
660nm
Ex/Em(nm)
Ex:650nm;Em:660nm
Grado
Azide Free, ExactAb™, Recombinant, RUO, Suitable for Flow Cytometry
Tipo de acción
ANTIBODY
Tipo de cadena ligera
kappa
Método de purificación
Protein A purified
Concentración
0.5 mg/mL
Almacenamiento y envío
Condiciones de almacenamiento de almacenamiento
Store at 2-8°C,Protected from light,Do not freeze
Enviado en
Wet ice,Do not freeze
Estabilidad y almacenamiento
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Flow cytometry analysis of CD8α expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD8α Antibody (APC) (Ab302183) at 0.1μg/test for 1 hour at 4°C. Left panel - Rat IgG (APC) (Ab180313).
Flow cytometry analysis of CD8α expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD8α Antibody (APC) (Ab302183) at 0.1μg/test for 1 hour at 4°C. Blue - Rat IgG (APC) (Ab180313). Black - Unlabelled control, cells without incubation with primary antibody.
Aplicación
Aplicación
Dilution info
Flow Cytometry
≤1.0 μg/test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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