Recombinant Human ACTA2 Protein, >90% (SDS-PAGE)

Cat. No.: rp156637

GRADE & PURITY Carrier Free, Azide Free, His Tag, ≥90%(SDS-PAGE)

Expression System

E. coli

Accession #

P62736

Protein Tag

N-His

Expression system

E. coli

Descripción general

Purity:>90%, by SDS-PAGE visualized with Coomassie® Blue Staining.
Description：
ACTA2 is a member of the actin family of proteins, an extremely conserved family of proteins which takes part in cell motility, structure and integrity. Three types of actin isoforms are known: Alpha, beta and gamma. Alpha actins are a main factor of the contractile mechanism, and beta and gamma take part in the regulation of cell motility. ACTA2 is an alpha actin which is located in skeletal muscle. Mutations in ACTA2 cause aortic aneurysm familial thoracic type 6(AAT6). Various alternatively spliced variants, encoding the same protein were identified. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.

Specifications

Product Name

Recombinant Human ACTA2 Protein, >90% (SDS-PAGE)

Sinónimos

Alpha-actin-2 | Cell growth-inhibiting gene 46 protein | a actin | AAT6 | ACTA_HUMAN | ACTA2 | Actin alpha 2 smooth muscle aorta | Actin aortic smooth muscle | Actin | aortic smooth muscle | ACTSA | ACTVS | Alpha 2 actin | Alpha actin 2 | Alpha cardiac ac

Grado

Azide Free, Carrier Free, His Tag

Especificaciones y pureza

Carrier Free, Azide Free, His-Tag, ≥90%(SDS-PAGE)

Pureza

>90% (SDS-PAGE)

Sistema de expresión

E. coli

Aminoácidos

1-377 aa

Secuencia

MGHHHHHHMCEEEDSTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDEAGPSIVHRKCF

Etiqueta proteínica

N-His

Número de adhesión

P62736

Peso molecular previsto

43.0 kDa

SDS-PAGE

43.2 kDa, under reducing conditions

Tipo de molécula

Protein

Almacenamiento y envío

Forma

Lyophilized

Reconstitución

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at -20°C for 1 year. Avoid freeze / thaw cycle.

Imágenes

SDS-PAGE: Recombinant Human ACTA2 Protein (rp156637)

Recombinant Human ACTA2 Protein (rp156637) - SDS-PAGE
3 μg/lane of Recombinant Human ACTA2 Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining, showing a band at 43.2 kDa.