Recombinant Human C-Reactive Protein/CRP Protein, >95% (SDS-PAGE)

Cat. No.: rp169601

GRADE & PURITY Animal Free, Carrier Free, Bioactive, ActiBioPure™, Azide Free, His Tag, ≥95%(SDS-PAGE)

Expression System

HEK293

Accession #

P02741

Protein Tag

C-His

Expression system

HEK293

Endotoxin Concentration

<1.0 EU/μg

Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human C-Reactive Protein/CRP (rp169601) at 1.0 μg/mL can bind Recombinant CRP Antibody (Ab007977) with the EC50 of 18.28 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human C-Reactive Protein/CRP (rp169601) at 1.0 μg/mL can bind Recombinant CRP Antibody (Ab007978) with the EC50 of 13.86 ng/mL.

Descripción general

Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining
Description:
CRP is a member of the pentraxin family of proteins that are characterized by a cyclic pentameric structure. Human CRP gene encodes a 224 amino acids precursor. The mature human CRP protein has 206 amino acids that are non-covalently linked to form the pentameter. Human CRP shares 71% and 64% amino acid sequence homology with mouse and rat respectively. CRP, synthesized by hepatocytes, is a major acute phase serum protein in human. IL-6, IL-1 and glucocorticoids are the major inducer of the CRP gene. In response to infection, inflammation or tissue damage, the level of CRP in human serum can increase 1,000-fold within 24-48 hours. It will come back to base level of less than 1 μg/mL very fast. Human CRP is an acute-phase serum protein that plays a role in the first line in host innate host defense. Like other pentraxins, CRP exhibits Ca++‑dependent binding to ligands. Phosphocholine (PCh), a constituent of many bacterial and fungal walls, is a principal ligand of CRP. CRP also binds to the membrane of injured cells, membrane and nuclear components of necrotic and apoptotic cells. Upon binding with the ligands, CRP is recognized by C1q and initiates the activation of complement cascade. Ligand bound CRP also binds to Fc gamma RI and Fc gamma RIIa on phagocytes and activates phogocytotic responses. In addition to phogocytosis, CRP also can induce production of hydrogen peroxide and inflammatory cytokines, such as IL-1, IL-6 and TNF‑ alpha by monocytes. With these functions, human CRP is an important serum protein for anti-bacterial pathogen and clearance of damaged and apoptotic cells. However, in mouse, CRP is expressed at very low level and is not an acute phase reactant. Serum amyloid P component (SAP), another pentraxin, is the major acute phase serum protein in mice. It has been shown that high levels of CRP in humans is associated with an increased risk of cardiovascular diseases.

Specifications

Product Name

Recombinant Human C-Reactive Protein/CRP Protein, >95% (SDS-PAGE)

Sinónimos

C-Reactive Protein | C-reactive protein, pentraxin-related | pentraxin 1 | PTX1MGC149895 Pentraxin 1, short | C reactive protein | C reactive protein pentraxin related | C-reactive protein(1-205) | CRP | CRP_HUMAN | MGC88244 | Pentraxin 1 | PTX 1 | PTX1

Grado

ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, His Tag

Especificaciones y pureza

Animal Free, Carrier Free, Bioactive, ActiBioPure™, Azide Free, His-Tag, ≥95%(SDS-PAGE)

Pureza

>95% (SDS-PAGE)

Bioactividad

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human C-Reactive Protein/CRP (rp169601) at 1.0 μg/mL can bind Recombinant CRP Antibody (Ab007977) with the EC50 of 18.28 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human C-Reactive Protein/CRP (rp169601) at 1.0 μg/mL can bind Recombinant CRP Antibody (Ab007978) with the EC50 of 13.86 ng/mL.

Concentración de endotoxinas

<1.0 EU/μg

Sistema de expresión

HEK293

Aminoácidos

1-224 aa

Secuencia

MEKLLCFLVLTSLSHAFGQTDMSRKAFVFPKESDTSYVSLKAPLTKPLKAFTVCLHFYTELSSTRGYSIFSYATKRQDNEILIFWSKDIGYSFTVGGSEILFEVPEVTVAPVHICTSWESASGIVEFWVDGKPRVRKSLKKGYTVGAEASIILGQEQDSFGGNFEGSQSLVGDIGNVNMWDFVLSPDEINTIYLGGPFSPNVLNWRALKYEVQGEVFTKPQLWPHHHHHH

Etiqueta proteínica

C-His

Número de adhesión

P02741

Peso molecular previsto

23.9 kDa

SDS-PAGE

24.8 kDa, under reducing conditions

Tipo de molécula

Protein

Almacenamiento y envío

Forma

Lyophilized

Reconstitución

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at -20°C stable up to 1 year. Avoid freeze / thaw cycle.

Imágenes

ELISA: Recombinant Human C-Reactive Protein/CRP Protein (rp169601)

Recombinant Human C-Reactive Protein/CRP Protein (rp169601) - ELISA
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human C-Reactive Protein/CRP (rp169601) at 1.0 μg/mL can bind Recombinant CRP Antibody (Ab007977) with the EC₅₀ of 18.28 ng/mL.

ELISA: Recombinant Human C-Reactive Protein/CRP Protein (rp169601)

Recombinant Human C-Reactive Protein/CRP Protein (rp169601) - ELISA
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human C-Reactive Protein/CRP (rp169601) at 1.0 μg/mL can bind Recombinant CRP Antibody (Ab007978) with the EC₅₀ of 13.86 ng/mL.

SDS-PAGE: Recombinant Human C-Reactive Protein/CRP Protein (rp169601)

Recombinant Human C-Reactive Protein/CRP Protein (rp169601) - SDS-PAGE
2 μg/lane of Recombinant Human C-Reactive Protein/CRP Protein was resolved with SDS-PAGE under reducing (R) conditions and visualized by Coomassie® Blue staining, showing a band at 24.8 kDa under reducing conditions.