GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.His Tag?His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein.≥90%(SDS-PAGE)See COA
Accession #
P00918
Protein Tag
C-10His
Expression system
E. coli
Endotoxin Concentration
<1.0 EU/μg
Bioactivity
Measured by its esterase activity. The specific activity is >100 pmol/min/µg, as measured under the described conditions.
Carrier Free,Bioactive,ActiBioPure™,High Performance,His-Tag,≥90%(SDS-PAGE),See COA ActiBioPure™,Bioactive,Carrier Free,High Performance,His Tag for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Specifications
Product Name
Recombinant Human Carbonic Anhydrase II Protein
Sinónimos
CA2 | CAII | CA-IIEC 4.2.1.1 | Car2 | Carbonate dehydratase II | carbonic anhydrase 2 | carbonic anhydrase B | Carbonic anhydrase C | Carbonic Anhydrase II | carbonic anhydrase IICAC | carbonic dehydratase
Grado
ActiBioPure™, Bioactive, Carrier Free, High Performance, His Tag
Especificaciones y pureza
Carrier Free, Bioactive, ActiBioPure™, High Performance, His-Tag, ≥90%(SDS-PAGE), See COA
Mecanismos bioquímicos y fisiológicos
Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification, and bone resorption. Topics in the CA meeting (6th International Conference on th
Bioactividad
Measured by its esterase activity. The specific activity is >100 pmol/min/µg, as measured under the described conditions.
29.2 kDa, under reducing conditions; 29.2 kDa, under non-reducing conditions.
Tipo de molécula
Protein
Almacenamiento y envío
Concentración
See COA
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
Imágenes
Recombinant Human Carbonic Anhydrase II Protein (rp183662) - SDS-PAGE 3 μg/lane of Recombinant Human Carbonic Anhydrase II Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 29.2 kDa.
Recombinant Human Carbonic Anhydrase II Protein- Enzyme Activity Measured by its esterase activity. The specific activity is >100 pmol/min/µg, as measured under the described conditions. Conditions: Assay Buffer: 12.5 mM Tris, 75 mM NaCl, pH 7.5 Recombinant Human Carbonic Anhydrase II Protein (rp183662) Substrate: 4-Nitrophenyl Acetate (4-NPA) (N111060) 1. Dilute rhCA2 to 2 ng/µL in Assay Buffer. 2. Dilute Substrate to 2 mM in Assay Buffer. 3. In a plate, load 50 µL of 2 ng/µL Recombinant Human Carbonic Anhydrase II Protein (rp183662), and start the reaction by adding 50 µL of 2 mM Substrate to wells. Include a Substrate Blank containing 50 µL Assay Buffer and 50 µL of 2 mM Substrate. 4. Read absorbance at a wavelength of 400 nm (bottom read) in kinetic mode for 5 minutes.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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