GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.His Tag?His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein.≥90%(SDS-PAGE)
Accession #
Q8N1Q1
Protein Tag
C-10His
Expression system
E. coli
Endotoxin Concentration
<1.0 EU/μg
Bioactivity
Measured by its esterase activity. The specific activity is >5 pmol/min/µg, as measured under the described conditions.
ActiBioPure™, Bioactive, Carrier Free, High Performance, His Tag
Especificaciones y pureza
Carrier Free, Bioactive, ActiBioPure™, High Performance, His-Tag, ≥90%(SDS-PAGE)
Mecanismos bioquímicos y fisiológicos
The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible
Bioactividad
Measured by its esterase activity. The specific activity is >5 pmol/min/µg, as measured under the described conditions.
30.5 kDa, under reducing conditions; 30.5 kDa, under non-reducing conditions.
Tipo de molécula
Protein
Almacenamiento y envío
Reconstitución
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
Imágenes
Recombinant Human Carbonic Anhydrase XIII Protein (rp183661) - SDS-PAGE 2 μg/lane of Recombinant Human Carbonic Anhydrase XIII Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 30.5 kDa.
Recombinant Human Carbonic Anhydrase XIII Protein- Enzyme Activity Measured by its esterase activity. The specific activity is >5 pmol/min/µg, as measured under the described conditions. Conditions: Assay Buffer: 12.5 mM Tris, 75 mM NaCl, pH 7.5 Recombinant Human Carbonic Anhydrase XIII Protein (rp183661) Substrate: 4-Nitrophenyl Acetate (4-NPA) (N111060), 100 mM stock in acetone 1.Dilute rhCA13 to 100 ng/µL in Assay Buffer. 2.Dilute Substrate to 2.0 mM in Assay Buffer. 3.In a plate load 50 µL of 100 ng/µL rhCA13, and include a Substrate Blank containing 50 µL Assay Buffer. 4.Start the reaction by adding 50 µL of 2.0 mM Substrate to wells containing rhCA13 and Assay Buffer. 5.Read at a wavelength of 400 nm (bottom read) in kinetic mode for 5 minutes.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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