Recombinant Human EGFR Isoform vIII Protein

Cat. No.: rp176275

GRADE & PURITY Animal Free, Carrier Free, Bioactive, ActiBioPure™, His Tag, PBS Only, ≥95%(SDS-PAGE)

Expression System

HEK293

Accession #

NP_001333870.1

Protein Tag

C-His

Expression system

HEK293

Endotoxin Concentration

<1.0 EU/μg

Bioactivity

Immobilized Recombinant Human EGFR Isoform vIII Protein (rp176275) at 1.0 μg/mL can bind Depatuxizumab mafodotin (anti-EGFR) (Ab175508) with the EC50 of 19.11 ng/mL. Immobilized Recombinant Human EGFR Isoform vIII Protein (rp176275) at 1.0 μg/mL can bind Depatuxizumab (anti-EGFR) (Ab182750) with the EC50 of 22.53 ng/mL.

Descripción general

Protein Function:
As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.

Specifications

Product Name

Recombinant Human EGFR Isoform vIII Protein

Sinónimos

avian erythroblastic leukemia viral (v-erb-b) oncogene homolog | cell growth inhibiting protein 40 | cell proliferation-inducing protein 61 | EC 2.7.10 | EC 2.7.10.1 | EGF R | EGFR | epidermal growth factor receptor (avian erythroblastic leukemia viral (v

Grado

ActiBioPure™, Animal Free, Bioactive, Carrier Free, His Tag, PBS Only

Especificaciones y pureza

Animal Free, Carrier Free, Bioactive, ActiBioPure™, His Tag, PBS Only, ≥95%(SDS-PAGE)

Bioactividad

Immobilized Recombinant Human EGFR Isoform vIII Protein (rp176275) at 1.0 μg/mL can bind Depatuxizumab mafodotin (anti-EGFR) (Ab175508) with the EC50 of 19.11 ng/mL. Immobilized Recombinant Human EGFR Isoform vIII Protein (rp176275) at 1.0 μg/mL can bind Depatuxizumab (anti-EGFR) (Ab182750) with the EC50 of 22.53 ng/mL.

Concentración de endotoxinas

<1.0 EU/μg

Sistema de expresión

HEK293

Aminoácidos

1-378 aa

Secuencia

MRPSGTAGAALLALLAALCPASRALEEKKGNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIPSHHHHHH

Etiqueta proteínica

C-His

Número de adhesión

NP_001333870.1

Peso molecular previsto

41.7 kDa

SDS-PAGE

56.2-67.2 kDa, under reducing conditions

Tipo de molécula

Protein

Almacenamiento y envío

Reconstitución

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at -20°C for 1 year. Avoid freeze / thaw cycle.

Imágenes

ELISA: Recombinant Human EGFR Isoform vIII Protein (rp176275)

Recombinant Human EGFR Isoform vIII Protein (rp176275) - ELISA
Immobilized Recombinant Human EGFR Isoform vIII Protein (rp176275) at 1.0 μg/mL can bind Depatuxizumab mafodotin (anti-EGFR) (Ab175508) with the EC50 of 19.11 ng/mL.

ELISA: Recombinant Human EGFR Isoform vIII Protein (rp176275)

Recombinant Human EGFR Isoform vIII Protein (rp176275) - ELISA
Immobilized Recombinant Human EGFR Isoform vIII Protein (rp176275) at 1.0 μg/mL can bind Depatuxizumab (anti-EGFR) (Ab182750) with the EC50 of 22.53 ng/mL.

SDS-PAGE: Recombinant Human EGFR Isoform vIII Protein (rp176275)

Recombinant Human EGFR Isoform vIII Protein (rp176275) - SDS-PAGE
3 μg/lane of Recombinant Human EGFR Isoform vIII Protein was resolved with SDS-PAGE under reducing (R) conditions and visualized by Coomassie® Blue staining, showing a band at 56.2-67.2 kDa under reducing conditions.