GRADE & PURITYAnimal Free?Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material.Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.His Tag?His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein.≥95%(SDS-PAGE)
Expression System
HEK293
Accession #
P98073
Protein Tag
C-9His
Expression system
HEK293
Endotoxin Concentration
<0.1 EU/μg
Bioactivity
Measured by its ability to cleave a colorimetric peptide substrate, Z-Lys-SBzl. The specific activity is >30,000 pmol/min/μg, as measured under the described conditions.
ActiBioPure™, Animal Free, Bioactive, Carrier Free, His Tag
Especificaciones y pureza
Animal Free, Carrier Free, Bioactive, ActiBioPure™, His Tag, ≥95%(SDS-PAGE)
Mecanismos bioquímicos y fisiológicos
Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarbox
Bioactividad
Measured by its ability to cleave a colorimetric peptide substrate, Z-Lys-SBzl. The specific activity is >30, 000 pmol/min/μg, as measured under the described conditions.
Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at -20~-80℃ for more than 1 year. Upon delivery aliquot. Avoid freeze/thaw cycle.
Imágenes
Recombinant Human Enteropeptidase/Enterokinase Protein (rp145565) - SDS-PAGE
2 μg/lane of Recombinant Human Enteropeptidase/Enterokinase Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 46 - 49 (light chain) & 130 - 150 (heavy chain) kDa under reducing conditions and 120 - 150 kDa under non-reducing conditions.
Human enterokinase is synthesized as a single-chain precursor. It must undergo an autocatalytic or intermolecular cleavage to remove a linker peptide, thereby converting into the mature and active heterodimer. This functional form consists of a heavy chain and a light chain connected by a disulfide bond. The simultaneous presence of both the uncleaved precursor and the mature heterodimer in the sample indicates that the recombinant protein preparation is a partially processed mixture.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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