Recombinant Human IGF-I/IGF-1 Protein

Cat. No.: rp228953

GRADE & PURITY ≥95%(SDS-PAGE), See COA

Accession #

P05019

Protein Tag

No tag

Expression system

E. coli

Endotoxin Concentration

<1.0 EU/μg

Bioactivity

Measured in a cell proliferation assay using MCF-7 human breast cancer cell line. The ED50 for this effect is typically 34.3 ng/mL.

Specifications

Product Name

Recombinant Human IGF-I/IGF-1 Protein

Sinónimos

IBP1 | IGF1 | IGF-1 | IGF1A | IGFI | IGF-I | IGF-IA | IGF-IB | insulin-like growth factor 1 (somatomedin C) | insulin-like growth factor 1 | insulin-like growth factor I | insulin-like growth factor IA | insulin-like growth factor IB | Mechano growth fact

Especificaciones y pureza

≥95%(SDS-PAGE), See COA

Mecanismos bioquímicos y fisiológicos

Insulin-like growth factor 1 (IGF-1 or IGF-I), also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Human IGF-1 is synthesized as two precursor isoforms with N- and alternate Cterminal prop

Bioactividad

Measured in a cell proliferation assay using MCF-7 human breast cancer cell line. The ED50 for this effect is typically 34.3 ng/mL.

Concentración de endotoxinas

<1.0 EU/μg

Aminoácidos

49-118 aa

Secuencia

GPETLCGAELVDALQFVCGDRGFYFNKPTGYGSSSRRAPQTGIVDECCFRSCDLRRLEMYCAPLKPAKSA

Número de adhesión

P05019

Peso molecular previsto

7.7 kDa

SDS-PAGE

7.6 kDa, under reducing conditions; 7.6 & 15.7, under non-reducing conditions.

Tipo de molécula

Protein

Almacenamiento y envío

Concentración

See COA

Reconstitución

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at -20℃ for 12 months. Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Protein Bioactivity: Recombinant Human IGF-I/IGF-1 Protein (rp228953)

Recombinant Human IGF-I/IGF-1 Protein (rp228953) - Protein Bioactivity

 Measured in a cell proliferation assay using MCF-7 human breast cancer cell line. The ED₅₀ for this effect is typically 9.08 ng/mL.

SDS-PAGE: Recombinant Human IGF-I/IGF-1 Protein (rp228953)

Recombinant Human IGF-I/IGF-1 Protein (rp228953) - SDS-PAGE 

3 μg/lane of Recombinant Human IGF-I/IGF-1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the bands at 7.6 kDa under reducing conditions and 7.6 & 15.7 kDa under non-reducing conditions. IGF-I/IGF-1 can form a stable dimer on its own, which is mediated by a disulfide bond connecting the C-terminal helices of the α-subunits (referred to as α-CT for short) on the two monomers (PMID: 31594955).