Recombinant Human LRPAP1 Protein

Cat. No.: rp184959

GRADE & PURITY Carrier Free, His Tag, PBS Only, ≥90%(SDS-PAGE)

Expression System

E. coli

Accession #

P30533

Protein Tag

C-His

Expression system

E. coli

Endotoxin Concentration

<1.0 EU/μg

Bioactivity

Testing in progress

Descripción general

Receptor-associated protein (RAP) is a molecular chaperone for low-density lipoprotein receptor-related protein (LRP), which plays a key role in cholesterol metabolism. The lipoprotein receptor-related protein (LRP) is an endocytic receptor for several ligands, such as alpha2-macroglobulin (alpha2 M) and apolipoprotein E. LRP is involved in the clearance of lipids from the bloodstream and is expressed in the atherosclerotic plaque. The LRP-associated protein (LRPAP in humans, RAP in mice) acts as a chaperone protein, stabilizing the nascent LRP peptide in the endoplasmic reticulum and Golgi complex. Alpha-2-macroglobulin receptor-associated protein, also known as low-density lipoprotein receptor-related protein-associated protein 1, RAP, and LRPAP1, is a 39 kDa protein and a member of the alpha-2-MRAP family. It is a receptor antagonist that interacts with several members of the low-density lipoprotein (LDL) receptor gene family. Upon binding to these receptors, LRPAP1 inhibits all ligand interactions with the receptors. LRPAP1 is present on the cell surface forming a complex with the alpha-2-macroglobulin receptor heavy and light chains. It binds with LRP1B and the binding is followed by internalization and degradation. LRPAP1 interacts with LRP1/alpha-2-macroglobulin receptor and LRP2 (previously called glycoprotein 330) and may be involved in the pathogenesis of membrane glomerular nephritis. LRPAP1 together with LRP2 forms the Heymann nephritis antigenic complex. LRP2 is expressed in epithelial cells of the thyroid, where it can serve as a receptor for the protein thyroglobulin. Intron 5 insertion/deletion polymorphism of RAP gene (LRPAP1) has been implicated in other diseases sharing etiology with gallstone disease (GSD). The LRPAP1 insertion/deletion polymorphism influences cholesterol homeostasis and may confer risk for gallstone disease and gallbladder carcinoma (GBC) incidence usually parallels with the prevalence of cholelithiasis. The genetic variations at the LRPAP1 locus may modulate Alzheimer's disease (AD) phenotype beyond risk for disease. Also, the variation in the LRPAP1 gene could contribute to the risk of developing an early episode of myocardial infarction (MI). 

Specifications

Product Name

Recombinant Human LRPAP1 Protein

Sinónimos

A2MRAP | A2MRAPMRAP | A2RAP | alpha-2-macroglobulin receptor-associated protein 1 | alpha-2-macroglobulin receptor-associated protein | alpha-2-MRAP | HBP44 | lipoprotein receptor associated protein | low density lipoprotein receptor-related protein assoc

Grado

Carrier Free, His Tag, PBS Only

Especificaciones y pureza

Carrier Free, His Tag, PBS Only, ≥90%(SDS-PAGE)

Bioactividad

Testing in progress

Concentración de endotoxinas

<1.0 EU/μg

Sistema de expresión

E. coli

Aminoácidos

35-357 aa

Secuencia

MYSREKNQPKPSPKRESGEEFRMEKLNQLWEKAQRLHLPPVRLAELHADLKIQERDELAWKKLKLDGLDEDGEKEARLIRNLNVILAKYGLDGKKDARQVTSNSLSGTQEDGLDDPRLEKLWHKAKTSGKFSGEELDKLWREFLHHKEKVHEYNVLLETLSRTEEIHENVISPSDLSDIKGSVLHSRHTELKEKLRSINQGLDRLRRVSHQGYSTEAEFEEPRVIDLWDLAQSANLTDKELEAFREELKHFEAKIEKHNHYQKQLEIAHEKLRHAESVGDGERVSRSREKHALLEGRTKELGYTVKKHLQDLSGRISRARHNELHHHHHH

Etiqueta proteínica

C-His

Número de adhesión

P30533

Peso molecular previsto

38.7 kDa

SDS-PAGE

41.8 kDa, under reducing conditions; 41.8 kDa, under non-reducing conditions

Tipo de molécula

Protein

Almacenamiento y envío

Reconstitución

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at -20°C for 1 year. Avoid freeze / thaw cycle.

Imágenes

SDS-PAGE: Recombinant Human LRPAP1 Protein (rp184959)

Recombinant Human LRPAP1 Protein (rp184959) - SDS-PAGE
1.5 μg/lane of Recombinant Human LRPAP1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 41.8 kDa.