GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.His Tag?His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)
Accession #
P14174
Protein Tag
N-8His
Expression system
E. coli
Endotoxin Concentration
<1.0 EU/μg
Bioactivity
Immobilized Recombinant Human MIF Protein (rp214395) at 1.0 μg/mL can bind Recombinant MIF Antibody (Ab115362) with the EC50 of 4.3 ng/mL.
ActiBioPure™, Bioactive, Carrier Free, His Tag, PBS Only
Especificaciones y pureza
Carrier Free, Bioactive, ActiBioPure™, His Tag, PBS Only, ≥95%(SDS-PAGE)
Mecanismos bioquímicos y fisiológicos
MIF (or macrophage migration inhibitory factor) was the first lymphokine/cytokine to be recognized in the pregenomics era. Regardless, it is one of the least understood of all inflammatory mediators. Human MIF is a 12.5 kDa, 115 amino acid (aa) nonglycosy
Bioactividad
Immobilized Recombinant Human MIF Protein (rp214395) at 1.0 μg/mL can bind Recombinant MIF Antibody (Ab115362) with the EC50 of 4.3 ng/mL.
12.0 kDa, under reducing conditions; 12.0 kDa, under non-reducing conditions.
Tipo de molécula
Protein
Almacenamiento y envío
Reconstitución
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
Imágenes
Recombinant Human MIF Protein (rp214395) - ELISA Immobilized Recombinant Human MIF Protein (rp214395) at 1.0 μg/mL can bind Recombinant MIF Antibody (Ab115362) with the EC50 of 4.3 ng/mL.
Recombinant Human MIF Protein (rp214395) - SDS-PAGE 3 μg/lane of Recombinant Human MIF Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 12.0 kDa.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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