GRADE & PURITYAnimal Free?Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material.Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.His Tag?His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein.≥90%(SDS-PAGE)
Expression System
HEK293
Accession #
P10451.1
Protein Tag
C-His
Expression system
HEK293
Endotoxin Concentration
<0.1 EU/μg
Bioactivity
1、Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells.Recombinant Human Osteopontin/OPN coated plate, cell adhesion is enhanced in a dose-dependent manner after 1 hour incubation at 37 °C. The ED₅₀ for this effect is 0.2-0.8 µg/mL. 2、Measured by its binding ability in a functional ELISA. When Recombinant Human Osteopontin (rp149597) is immobilized at 4 µg/mL, biotinylated Recombinant Human Mesothelin (rp176301) binds with an ED50 of 40.2 ng/mL.
Animal Free,Carrier Free,Bioactive,ActiBioPure™,Azide Free,High Performance,His-Tag,≥90%(SDS-PAGE) ActiBioPure™,Animal Free,Azide Free,Bioactive,Carrier Free,High Performance,His Tag for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Purity
>90% SDS-PAGE.
Function
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
Post-translational
Extensively phosphorylated on clustered serine residues. N- and O-glycosylated. Phosphorylation sites are present in the extracelllular medium.
Specifications
Product Name
Recombinant Human Osteopontin Protein, ＞90%(SDS-PAGE)
Sinónimos
BNSP | Bone sialoprotein 1 | Bone sialoprotein I | BSP I | BSPI | Early T lymphocyte activation 1 | ETA 1 | ETA1 | MGC110940 | Nephropontin | OPN | Osteopontin | osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein | OSTP_HUMAN |
Grado
ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance, His Tag
1、Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells.Recombinant Human Osteopontin/OPN coated plate, cell adhesion is enhanced in a dose-dependent manner after 1 hour incubation at 37 °C. The ED₅₀ for this effect is 0.2-0.8 µg/mL. 2、Measured by its binding ability in a functional ELISA. When Recombinant Human Osteopontin (rp149597) is immobilized at 4 µg/mL, biotinylated Recombinant Human Mesothelin (rp176301) binds with an ED50 of 40.2 ng/mL.
59.7 & 17.0 & 13.1 kDa, under reducing conditions; 59.7 & 17.0 & 13.1 kDa, under non-reducing conditions.
Tipo de molécula
Protein
Almacenamiento y envío
Forma
Lyophilized
Reconstitución
Reconstitute in water to a concentration of 0.1-0.5 mg/ml.
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at -20~-80℃ for more than 1 year
Imágenes
Recombinant Human Osteopontin Protein (rp149597) - Protein Bioactivity Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. Recombinant Human Osteopontin Protein (rp149597) coated plate, cell adhesion is enhanced in a dose-dependent manner after 1 hour incubation at 37℃. The ED₅₀ for this effect is 0.2 - 0.8 µg/mL.
Recombinant Human Osteopontin Protein (rp149597) - Protein Bioactivity Measured by its binding ability in a functional ELISA. When Recombinant Human Osteopontin (rp149597) is immobilized at 4 µg/mL, biotinylated Recombinant Human Mesothelin (rp176301) binds with an ED₅₀ of 40.2 ng/mL.
Recombinant Human Osteopontin Protein (rp149597) - SDS-PAGE 3 μg/lane of Recombinant Human Osteopontin Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 59.7 & 17.0 & 13.1 kDa.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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