GRADE & PURITYAnimal Free?Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material.Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.His Tag?His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein.≥95%(SDS-PAGE)
Expression System
HEK293
Accession #
Q96B86
Protein Tag
C-His
Expression system
HEK293
Endotoxin Concentration
<0.1 EU/μg
Bioactivity
Measured by its binding ability in a functional ELISA. When rhBMP-4 is coated at 1 μg/mL, rhRGM-A binds with an apparent KD <50nM. Immobilized rhRGM-A can bind rmNeogenin/Fc Chimera with a linear range of 10-500 ng/mL.
ActiBioPure™, bioactivo, sin animales, sin portador, ≥95%(SDS-PAGE) ActiBioPure™,Animal Free,Bioactive,Carrier Free,His Tag for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
🌡
Storage & shipping
Conservar a -20°C,Evitar la congelación y descongelación repetidas Ships Hielera + almohadillas de hielo Check lot-specific COA for exact specifications.
📋
Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
📚
Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Función Miembro de la familia de las moléculas guía repulsivas (RGM) que desempeña varias funciones en el sistema nervioso en desarrollo y adulto. Regula el cierre del tubo neural cefálico, inhibe el crecimiento de neuritas y la ramificación de neuronas corticales, así como la formación de sinapsis maduras. La unión a su receptor NEO1/neogenina induce la activación de la vía de señalización RHOA-ROCK1/Rho-quinasa a través de la cascada UNC5B-ARHGEF12/LARG-PTK2/FAK1, lo que provoca el colapso del cono de crecimiento neuronal y la inhibición del crecimiento de las neuritas. Además, la unión de RGMA a NEO1/neogenina conduce a la inactivación de HRAS al influir en la vía HRAS1-PTK2/FAK1-AKT1. También funciona como un coreceptor de la proteína morfogenética ósea (BMP) que puede señalar a través de SMAD1, SMAD5 y SMAD8.
Specifications
Product Name
Proteína humana recombinante RGM-A
Sinónimos
Familia de dominios RGM, miembro A | Molécula de guía repulsiva A | Familia de dominios RGM, miembro A | RGMA | RGM-A | Molécula de guía repulsiva A | RGM
Grado
ActiBioPure™, Animal Free, Bioactive, Carrier Free, His Tag
Especificaciones y pureza
ActiBioPure™, bioactivo, sin animales, sin portador, ≥95%(SDS-PAGE)
Bioactividad
Measured by its binding ability in a functional ELISA. When rhBMP-4 is coated at 1 μg/mL, rhRGM-A binds with an apparent KD <50nM. Immobilized rhRGM-A can bind rmNeogenin/Fc Chimera with a linear range of 10-500 ng/mL.
Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
Condiciones de almacenamiento de almacenamiento
Conservar a -20°C,Evitar la congelación y descongelación repetidas
Enviado en
Hielera + almohadillas de hielo
Estabilidad y almacenamiento
Almacenar a -20~-80℃ durante más de 1 año. A la entrega alícuota. Evite el ciclo de congelación/descongelación.
Imágenes
Recombinant Human RGM-A Protein (rp150956) - SDS-PAGE 2 μg/lane of Recombinant Human RGM-A Proteinwas resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 33 & 27 kDa under reducing conditions and 53 kDa under non-reducing conditions. Human Repulsive Guidance molecule (RGM) is a 33 kDa GPI-linked member of an expanding RGM-related family of neuronal and muscle-expressed membrane proteins. It is synthesized as a 450 amino acid (aa) preproprotein that contains a 47 aa signal sequence, a 121 aa N-terminal prosegment, a 256 mature region and a 26 aa C-terminal prosegment (PMID: 14985445,12353034).
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
We use cookies to ensure the website functions properly and, where permitted, to improve your experience. You can manage your preferences at any time in Settings. Learn more in our Cookie Policy.
Shall we send you a message when we have discounts available?
Remind me later
Thank you! Please check your email inbox to confirm.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
ZJ25F0522100