Recombinant Human TRAIL Protein

Cat. No.: rp169692

GRADE & PURITY Carrier Free, Bioactive, ActiBioPure™, Azide Free, High Performance, His Tag, ≥90%(SDS-PAGE)

Expression System

E. coli

Accession #

P50591

Protein Tag

N-His

Expression system

E. coli

Endotoxin Concentration

<1.0 EU/μg

Bioactivity

Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 12.76 ng/mL.

Descripción general

Purity:>90%, by SDS-PAGE visualized with Coomassie® Blue Staining.
Description：
TRAIL (TNF-related apoptosis-inducing ligand), also known as APO-2 ligand and TNFSF10, is a type II transmembrane protein with a carboxy-terminal extracellular domain which exhibits homology to other TNF family members. In the TNF superfamily nomenclature, TRAIL is referred to as TNFSF10. Human TRAIL cDNA encodes a 281 amino acid (aa) residue protein with an amino-terminal intracellular domain of 17 residues and a predicted internal hydrophobic domain between residues 18 and 38. The extracellular carboxy-terminal domain contains a potential N-linked glycosylation site at amino acid residue 109. Among TNF family members, TRAIL is the most homologous to Fas ligand, sharing 28% aa sequence identity in their extracellular domains. Mouse TRAIL has also been cloned. The human TRAIL shares 65% aa sequence identity with mouse TRAIL and is active on mouse cells. Both membrane bound and soluble TRAIL have been shown to induce rapid apoptosis of many transformed cell lines. Like most TNF family members, the bioactive TRAIL exists as a homotrimer. TRAIL transcripts have been shown to be constitutively expressed in a variety of human tissues. A family of TRAIL receptors, including two receptors that transduce the apoptotic signals and two TRAIL decoy receptors that function to antagonize TRAIL-induced apoptosis, have been identified. Osteoprotegerin has been identified as a fifth TRAIL receptor. It was shown that homotrimeric TRAIL binds a zinc ion which is critical for the correct structure of the protein.

Specifications

Product Name

Recombinant Human TRAIL Protein

Sinónimos

Apo-2 ligand | APO2L | Apo-2Ltumor necrosis factor (ligand) family, member 10 | APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta | CD253 antigen | CD253 | Protein TRAIL | TL2 | TNF-related apoptosis-inducing ligand | TNFSF10 | TRA

Grado

ActiBioPure™, Azide Free, Bioactive, Carrier Free, High Performance, His Tag

Especificaciones y pureza

Carrier Free, Bioactive, ActiBioPure™, Azide Free, High Performance, His-Tag, ≥90%(SDS-PAGE)

Bioactividad

Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 12.76 ng/mL.

Concentración de endotoxinas

<1.0 EU/μg

Sistema de expresión

E. coli

Aminoácidos

114-281aa

Secuencia

MHHHHHHVRERGPQRVAAHITGTRGRSNTLSSPNSKNEKALGRKINSWESSRSGHSFLSNLHLRNGELVIHEKGFYYIYSQTYFRFQEEIKENTKNDKQMVQYIYKYTSYPDPILLMKSARNSCWSKDAEYGLYSIYQGGIFELKENDRIFVSVTNEHLIDMDHEASFFGAFLVG

Etiqueta proteínica

N-His

Número de adhesión

P50591

Peso molecular previsto

20.4 kDa

SDS-PAGE

18.7 kDa and 40.0 kDa, reducing conditions; 18.7 kDa, 40.0 kDa and 59.3 kDa, non-reducing conditions

Tipo de molécula

Protein

Almacenamiento y envío

Forma

Transparent gelatinous

Reconstitución

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at -20°C stable up to 1 year. Avoid freeze/thaw cycle.

Imágenes

Protein Bioactivity: Recombinant Human TRAIL/TNFSF10 Protein (rp169692)

Recombinant Human TRAIL/TNFSF10 Protein (rp169692) - Protein Bioactivity
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D (A113142). The ED50 for this effect is typically less than 20.0 ng/mL.

SDS-PAGE: Recombinant Human TRAIL/TNFSF10 Protein (rp169692)

Recombinant Human TRAIL/TNFSF10 Protein (rp169692) - SDS-PAGE
Recombinant Human TRAIL/TNFSF10 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing two bands at 18.7 and 40.0 kDa under reducing conditions, three bands at 18.7, 40.0 and 59.3 kDa under non-reducing conditions.