GRADE & PURITYAnimal Free?Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material.Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.His Tag?His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)
Expression System
HEK293
Accession #
O14763
Protein Tag
C-His
Expression system
HEK293
Endotoxin Concentration
<1.0 EU/μg
Bioactivity
Recombinant Human TRAIL R2/TNFRSF10B Protein (rp176693) inhibits Recombinant Human TRAIL mediated cytotoxicity using L-929 mouse fibroblast cells. The ED50 for this effect is 27.85 ng/mL. Immobilized Recombinant Human TRAIL R2/TNFRSF10B Protein (rp176693) at 5.0 μg/mL can bind Recombinant Human TRAIL Protein (rp169692) with the EC50 of 41.55 ng/mL.
Animal Free,Carrier Free,Bioactive,High Performance,His Tag,PBS Only,≥95%(SDS-PAGE) Animal Free,Bioactive,Carrier Free,High Performance,His Tag,PBS Only for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Purity: ≥95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： Tumor necrosis factor receptor superfamily, member 10b, official symbol TNFRSF10B, also known as Death receptor 5, CD262, TNF-related apoptosis-inducing ligand receptor 2 (TRAIL R2), is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. TRAIL R2/CD262/TNFRSF10B was purified independently as the only receptor for TRAIL detectable on the surface of two different human cell lines that undergo apoptosis upon stimulation with TRAIL. TRAIL R2/CD262/TNFRSF10B contains two extracellular cysteine-rich repeats, typical for TNF receptor (TNFR) family members, and a cytoplasmic death domain. TRAIL R2/CD262/TNFRSF10B mediates apoptosis via the intracellular adaptor molecule FADD/MORT1. TRAIL receptors can signal both death and gene transcription, functions reminiscent of those of TNFR1 and TRAMP, two other members of the death receptor family. Defects in TRAIL R2/CD262/TNFRSF10B may be a cause of head and neck squamous cell carcinomas (HNSCC) also known as squamous cell carcinoma of the head and neck.
Specifications
Product Name
Recombinant Human TRAIL R2/TNFRSF10B Protein
Sinónimos
CD262 antigen | CD262 | death domain containing receptor for TRAIL/Apo-2L | Death receptor 5 | DR5 | DR5TRICK2B | Fas-like protein | KILLER/DR5 | KILLERapoptosis inducing protein TRICK2A/2B | TNF-related apoptosis-inducing ligand receptor 2 | TNFRSF10B |
Grado
Animal Free, Bioactive, Carrier Free, High Performance, His Tag, PBS Only
Especificaciones y pureza
Animal Free, Carrier Free, Bioactive, High Performance, His Tag, PBS Only, ≥95%(SDS-PAGE)
Bioactividad
Recombinant Human TRAIL R2/TNFRSF10B Protein (rp176693) inhibits Recombinant Human TRAIL mediated cytotoxicity using L-929 mouse fibroblast cells. The ED50 for this effect is 27.85 ng/mL. Immobilized Recombinant Human TRAIL R2/TNFRSF10B Protein (rp176693) at 5.0 μg/mL can bind Recombinant Human TRAIL Protein (rp169692) with the EC50 of 41.55 ng/mL.
14.7&18.7 kDa, under reducing conditions; 13.7&17.5 kDa, under non-reducing conditions
Tipo de molécula
Protein
Almacenamiento y envío
Forma
Lyophilized
Reconstitución
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at -20°C for 1 year. Avoid freeze / thaw cycle.
Imágenes
Recombinant Human TRAIL R2/TNFRSF10B Protein (rp176693)- Protein Bioactivity
Recombinant Human TRAIL R2/TNFRSF10B Protein (rp176693) inhibits Recombinant Human TRAIL mediated cytotoxicity using L-929 mouse fibroblast cells. The ED₅₀ for this effect is 27.85 ng/mL.
Recombinant Human TRAIL R2/TNFRSF10B Protein (rp176693) - ELISA Immobilized Recombinant Human TRAIL R2/TNFRSF10B Protein (rp176693) at 5.0 μg/mL can bind Recombinant Human TRAIL Protein (rp169692) with the EC₅₀ of 41.55 ng/mL.
Recombinant Human TRAIL R2/TNFRSF10B Protein (rp176693) - SDS-PAGE 3 μg/lane of Recombinant Human TRAIL R2/TNFRSF10B Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 14.7&18.7 kDa under reducing conditions and 13.7&17.5 kDa under non-reducing conditions.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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