Recombinant Human YKL-40/CHI3L1 Protein

Cat. No.: rp169594

GRADE & PURITY Animal Free, Carrier Free, His Tag, PBS Only, ≥95%(SDS-PAGE)

Expression System

HEK293

Accession #

P36222

Protein Tag

C-His

Expression system

HEK293

Endotoxin Concentration

<1.0 EU/μg

Bioactivity

Testing in progress

Descripción general

Protein Function：
Chitinase 3-like 1 (CHI3L1), also called YKL-40 or human cartilage glycoprotein 39 (HCGP39), is a 39 kDa glycoprotein member of the glycosyl hydrolase 18 family. CHI3L1 was first identified as secreted from cultured articular chondrocytes, synovial cells, and activated monocyte-derived macrophages, but it is also secreted by neutrophils, endothelial cells, vascular smooth muscle cells, and some cancer cells. The human CHI3L1 cDNA encodes 383 amino acids (aa), including a 21 aa signal sequence and a 362 aa mature region with two intramolecular disulfides. Mature human CHI3L1 shares 73%, 80%, 82%, 83%, 86% and 87% aa sequence identity with mouse, rat, canine, bovine, porcine and equine CHI3L1, respectively. Human CHI3L1 and CHI3L2 share 43% aa sequence identity. Neither shows chitotriosidase activity, but both bind chitin and are thus termed chi-lectins. CHI3L1 can bind heparins, probably as heparan sulfate. It has been found to enhance cell adhesion and promote cell signaling, proliferation and tumor angiogenesis. Elevated serum CHI3L1 levels occur in some conditions characterized by inflammation and connective tissue remodeling, such as arthritis, chronic obstructive pulmonary disease, diabetes, cardiovascular disease, inflammatory bowel disease, and liver cirrhosis. Single nucleotide polymorphisms that can increase serum CHI3L1 are associated with higher risk for asthma in childhood. CHI3L1 is frequently upregulated in glioblastoma, myxoid chondrosarcoma, melanoma and carcinomas of the breast, thyroid, colon, lung, kidney, and ovary. In asthma and cancer, serum CHI3L1 concentrations can correlate with prognosis.

Specifications

Product Name

Recombinant Human YKL-40/CHI3L1 Protein

Sinónimos

39 kDa synovial protein | ASRT7 | Cartilage glycoprotein 39 | CGP-39 | CGP39 | CH3L1_HUMAN | CHI3L1 | chitinase | chitinase 3 like 1 | chitinase 3 like 1 (cartilage glycoprotein 39) | Chitinase 3 like protein 1 precursor | Chitinase-3-like protein 1 | Cho

Grado

Animal Free, Carrier Free, His Tag, PBS Only

Especificaciones y pureza

Animal Free, Carrier Free, His Tag, PBS Only, ≥95%(SDS-PAGE)

Bioactividad

Testing in progress

Concentración de endotoxinas

<1.0 EU/μg

Sistema de expresión

HEK293

Aminoácidos

1-383 aa

Secuencia

MGVKASQTGFVVLVLLQCCSAYKLVCYYTSWSQYREGDGSCFPDALDRFLCTHIIYSFANISNDHIDTWEWNDVTLYGMLNTLKNRNPNLKTLLSVGGWNFGSQRFSKIASNTQSRRTFIKSVPPFLRTHGFDGLDLAWLYPGRRDKQHFTTLIKEMKAEFIKEAQPGKKQLLLSAALSAGKVTIDSSYDIAKISQHLDFISIMTYDFHGAWRGTTGHHSPLFRGQEDASPDRFSNTDYAVGYMLRLGAPASKLVMGIPTFGRSFTLASSETGVGAPISGPGIPGRFTKEAGTLAYYEICDFLRGATVHRILGQQVPYATKGNQWVGYDDQESVKSKVQYLKDRQLAGAMVWALDLDDFQGSFCGQDLRFPLTNAIKDALAATHHHHHH

Etiqueta proteínica

C-His

Número de adhesión

P36222

Peso molecular previsto

43.6 kDa

SDS-PAGE

42.3 kDa, under reducing condition; 42.1 & 79.5 kDa, under non-reducing condition

Tipo de molécula

Protein

Almacenamiento y envío

Reconstitución

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at -20°C for 1 year. Avoid freeze / thaw cycle.

Imágenes

SDS-PAGE: Recombinant Human YKL-40/CHI3L1 Protein (rp169594)

Recombinant Human YKL-40/CHI3L1 Protein (rp169594) - SDS-PAGE
3 μg/lane of Recombinant Human YKL-40/CHI3L1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 42.3 kDa under reducing conditions and 42.1 & 79.5 kDa under non-reducing conditions.