Recombinant Streptavidin Protein (AF594)

Cat. No.: rp181945

GRADE & PURITY Validated, ExactAb™, Ex:590nm, Em:618nm, 1.0 mg/mL

Accession #

P22629

Expression system

E. coli

Bioactivity

Binding Biotin

Specifications

Product Name

Recombinant Streptavidin Protein (AF594)

Sinónimos

SA V1 | SA V2 | strepavidin | Streptavidin V1 | Streptavidin V2 | Streptavidin-AF594 | AF594-Streptavidin | SA-AF594

Grado

Em:618nm, Ex:590nm, ExactAb™, Validated

Especificaciones y pureza

ExactAb™, Validated, Ex:590nm, Em:618nm, 1.0 mg/mL

Bioactividad

Binding Biotin

Conjugación

AF594

Excitación (nm)

Yellow-Green Laser(594nm)

Emisión (nm)

618nm

Ex/Em(nm)

Número de adhesión

P22629

Peso molecular previsto

19 kDa

Tipo de molécula

Protein

Almacenamiento y envío

Concentración

1.0 mg/mL

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Protected from light,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

The product should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imágenes

IF: Recombinant Streptavidin Protein (AF594) (rp181945)

Recombinant Streptavidin Protein (AF594) (rp181945) - IF
IF analysis of beta Tubulin (red) in Hela cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Then incubated with Recombinant Streptavidin Protein (AF594) (rp181945) at a dilution of 1/500 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: Recombinant Streptavidin Protein (AF594) (rp181945)

Recombinant Streptavidin Protein (AF594) (rp181945) - Flow Cytometry
Flow cytometry analysis of CD45 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with CD45 Mouse mAb (Biotin) (Ab179532) at 0.05μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (AF594) (rp181945) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

• Aplicación: Dilution info
• IF/ICC:

  1:500 ;

• Flow Cytometry:

  ≤1.0 µg/test; For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume. Since applications vary, you should determine the optimum working

Referencias

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