Recombinant Streptavidin Protein (PE)

Cat. No.: rp230629

GRADE & PURITY Carrier Free, Validated, ExactAb™, High Performance, 1.0 mg/mL

Expression system

E. coli

Bioactivity

Binding Biotin

Descripción general

Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. This Streptavidin-R-PE conjugate comprises streptavidin (as the biotin-binding protein) with RPE covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. It is a very valuable tool for biotin-streptavidin-based biological assays and tests using flow cytometry, microplate reader and microarray platforms. This Streptavidin-R-PE product has been tested by immunofluorescent staining with flow cytometric analysis and Multiplex bead-based assay.

Specifications

Product Name

Recombinant Streptavidin Protein (PE)

Sinónimos

SA V1 | SA V2 | strepavidin | Streptavidin V1 | Streptavidin V2 | Streptavidin | Streptavidin-R-PE | R-phycoerythrin conjugate | SA-PE | Streptavidin-Phycoerythrin | SAv-PE | RPE-streptavidin conjugate

Grado

Carrier Free, ExactAb™, High Performance, Validated

Especificaciones y pureza

Carrier Free, ExactAb™, Validated, High Performance, 1.0 mg/mL

Mecanismos bioquímicos y fisiológicos

Streptavidin is a biotin binding protein and has the ability to bind four molecules of biotin. The biotin binding pocket of streptavidin helps to interact with biotin. It can be used to enhance protein binding and multimerization.

Bioactividad

Binding Biotin

Conjugación

PE

Excitación (nm)

Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)

Emisión (nm)

575nm

Ex/Em(nm)

Tipo de molécula

Protein

Almacenamiento y envío

Concentración

1.0 mg/mL

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Protected from light,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

The product should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imágenes

Multiplex bead-based assay: Recombinant Streptavidin Protein (PE) (rp230629)

Recombinant Streptavidin Protein (PE) (rp230629) - Multiplex bead-based assay
Multiplex bead-based assay and High Performance assays use color-coded polystyrene or superparamagnetic beads coated with target-specific antibodies. When these pre-coated beads are incubated with the sample mixture, the target analytes are captured by the beads. These captured analytes are then detected by a cocktail of biotinylated detection antibodies. Following the addition of a streptavidin-PE reporter, chemiluminescence is generated and detected.
·Capture Antibody (Mouse IgG1): 3 μg/scale
· Detection Antibody (Biotin goat Anti-Mouse IgG1): 2500 ng/mL
· SA-PE dilution ratio: 1/100, 1/1000, 1/10000 and 1/100000
Recombinant Streptavidin Protein (PE) (rp230629) is an indispensable fluorescent reporter reagent in the Luminex multiplex detection platform. Relying on its high-affinity binding to biotin and excellent photoluminescent properties, it enables high-sensitivity multiplex analysis.
Aladdin has independently developed a series of streptavidin-PE products (rp230625, rp230626, rp230627, rp230628, rp230629 and rp230630) with distinct formulations, all of which demonstrate excellent performance in the Multiplex bead-based assay detection platform.

Multiplex bead-based assay: Recombinant Streptavidin Protein (PE) (rp230629)

Recombinant Streptavidin Protein (PE) (rp230629) - Multiplex bead-based assay
Multiplex bead-based assay and High Performance assays use color-coded polystyrene or superparamagnetic beads coated with target-specific antibodies. When these pre-coated beads are incubated with the sample mixture, the target analytes are captured by the beads. These captured analytes are then detected by a cocktail of biotinylated detection antibodies. Following the addition of a streptavidin-PE reporter, chemiluminescence is generated and detected.
·Capture Antibody (Mouse IgG1): 3 μg/scale
·Detection Antibody (Biotin goat Anti-Mouse IgG1): 10 ng/mL
·SA-PE dilution ratio: 1/100, 1/1000, 1/10000 and 1/100000
Recombinant Streptavidin Protein (PE) (rp230629) is an indispensable fluorescent reporter reagent in the Luminex multiplex detection platform. Relying on its high-affinity binding to biotin and excellent photoluminescent properties, it enables high-sensitivity multiplex analysis.
Aladdin has independently developed a series of streptavidin-PE products (rp230625, rp230626, rp230627, rp230628, rp230629 and rp230630) with distinct formulations, all of which demonstrate excellent performance in the Multiplex bead-based assay detection platform.

Flow Cytometry: Recombinant Streptavidin Protein (PE) (rp230629)

Recombinant Streptavidin Protein (PE) (rp230629) - Flow Cytometry
Flow cytometry analysis of CD45 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with Recombinant CD45 Antibody (Biotin) (Ab213118) at 0.5μg/mL for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (PE) (rp230629) at 0.3μg/mL for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.