Recombinant Streptavidin Protein (Texas red)

Cat. No.: S639037

GRADE & PURITY Validated, ExactAb™, 1.0 mg/mL

Expression system

E. coli

Bioactivity

Binding Biotin

Descripción general

Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays.

Specifications

Product Name

Recombinant Streptavidin Protein (Texas red)

Sinónimos

SA V1 | SA V2 | strepavidin | Streptavidin V1 | Streptavidin V2 | Streptavidin

Grado

ExactAb™, Validated

Especificaciones y pureza

ExactAb™, Validated, 1.0 mg/mL

Mecanismos bioquímicos y fisiológicos

Streptomycin is a biotin binding protein that can bind to four types of biotin molecules. The biotin binding pocket of streptavidin facilitates interaction with biotin. It can be used to enhance protein binding and polymerization.

Bioactividad

Binding Biotin

Conjugación

Texas Red ®

Excitación (nm)

Yellow-Green Laser(561nm)

Emisión (nm)

615nm

Ex/Em(nm)

Tipo de molécula

Protein

Almacenamiento y envío

Forma

Solid

Concentración

1.0 mg/mL

Condiciones de almacenamiento de almacenamiento

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at -20°C. Protect from light. Avoid freeze/thaw cycles.

Imágenes

IF/ICC: Recombinant Streptavidin Protein (Texas red) (S639037)

Recombinant Streptavidin Protein (Texas red) (S639037) – IF/ICC
IF analysis of EpCAM (red) in HT-29 cells. The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with biotinylated   Oportuzumab (anti-EpCAM) (Ab209902) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Recombinant Streptavidin Protein (Texas red) (S639037) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: Recombinant Streptavidin Protein (Texas red) (S639037)

Recombinant Streptavidin Protein (Texas red) (S639037) - Flow Cytometry
Flow cytometry analysis of CD45 expression by Jarkat cells (red) compared to an isotype control (blue). Cells were stained with Recombinant CD45 Antibody (Biotin) (Ab213118) at 0.05μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (Texas red) (S639037) at 1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

• Aplicación: Dilution info
• IF/ICC:

  1-10μg/mL; Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.

• Flow Cytometry:

  1.0 -10μg/mL; Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.;