RNA Sample Loading Buffer - for NA electrophoresis, without ethidium bromide

Cat. No.: R476174

GRADE & PURITY for NA electrophoresis, without ethidium bromide

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Descripción general

RNA loading buffer is used as a tracking dye during RNA electrophoresis. The dye has a slight negative charge and will migrate the same direction as RNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition. Dilute 1:3 to 1:6 with sample, heat to 65C for ten minutes and chill on ice before loading.Suitable for use with formaldehyde-agarose gels used in Northern blotting procedures.RNA sample loading buffer is especially formulated for electrophoresis of RNA on formaldehyde-agarose gels with or without ethidium bromide. Ethidium bromide is not recommended for gel staining prior to Northern blot detection because the presence of ethidium bromide in agarose gels or the loading buffer can cause poor transfer efficiency.

Specifications

Especificaciones y pureza

for NA electrophoresis, without ethidium bromide

Condiciones de almacenamiento de almacenamiento

Store at -20°C

Enviado en

Ice chest + Ice pads

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