RNasin

Cat. No.: R665506

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Descripción general

This product is a recombinant RNase inhibitor expressed and purified in soluble form in Escherichia coli, with a molecular weight of approximately 65 KDa and a broad-spectrum RNase inhibitor. RNasin can specifically bind to RNase in a non covalent bond to form a complex, thereby inactivating RNase, without inhibiting the activity of RNase H, S1 nuclease, SP6, T7 or T3 RNA polymerase, AMV or M-MLV reverse transcriptase, Taq DNA polymerase, RNaseT1 and other enzymes, without affecting subsequent reverse transcription and translation processes. Widely used in RNA research, such as RT-PCR, cDNA synthesis, mRNA protection, in vitro transcription and translation, preparation of RNase Free antibodies, in situ hybridization, and mRNA localization.

Component	R665506 30 μL	R665506 1 ml
RNasin (40 U/μL)	30 μL	1 ml

Storage buffer:
HEPES-KOH (pH 7.6) 20 mM, KCl 50 mM, DTT 8mM, Glycerol 50%.

Activity definition:
One active unit (U) refers to the amount of enzyme used to inhibit the hydrolysis of glycoside 2 ', 3' - cyclic phosphate in 50% 5ng RNase A.

Purity:
1.300 U of RNasin and 1 μ G's λ The DNA Hind III decomposition product reacted at 37 ℃ for 1 hour without any change in the electrophoresis band of DNA.
2.300 U of RNAsin and 1 μ The super helix pBR322 DNA of g reacted at 37 ℃ for 1 hour, and the electrophoresis band of the DNA did not change.
3.100 U of RNasin and 1 μ The 16S and 23S rRNAs of g reacted at 37 ℃ for 1 hour, and the electrophoresis bands of the RNA did not change.

Main uses：
1. cDNA synthesis.
2. In vitro translation.
3. In vitro transcription.
4. RNA amplification.
5. RNA purification and storage.

Notes:
1. This product should avoid repeated freeze-thaw cycles. Please store it at -70 ℃ for long-term storage.
2. It is recommended to use a final concentration of 1 U/ μ L.

Specifications

Condiciones de almacenamiento de almacenamiento

Store at -20°C

Enviado en

Ice chest + Ice pads

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