RPMI 1640 medium is named after the Roswell Park Memorial Institute (RPMI), where it was developed, with 1640 serving as its product code. It is an improved version of McCoy's 5A medium and adopts a bicarbonate buffer system. Initially developed for the suspension and monolayer culture of human leukemia cells, RPMI 1640 has since been proven suitable for a wide range of mammalian cells, including HeLa, Jurkat, MCF-7, PC-12, PBMCs, astrocytes and cancer cells. Particularly ideal for suspension cell culture, it ranks among the most widely used cell culture media worldwide.
Distinct from other culture media, RPMI 1640 contains reduced glutathione and high concentrations of vitamins. It comprises biotin, vitamin B12 and para-aminobenzoic acid, which are absent in EMEM and DMEM, as well as high levels of choline chloride and inositol.
L-alanyl-L-glutamine is a premium cell culture supplement that can directly replace L-glutamine. L-glutamine is an essential nutrient in liquid culture media, yet it is unstable in aqueous solutions and prone to degradation, producing ammonia that is toxic to cells. In contrast, L-alanyl-L-glutamine features excellent stability in water and does not degrade spontaneously. Its metabolic mechanism in cell culture is as follows: cells secrete peptidase into the culture medium, which gradually hydrolyzes L-alanyl-L-glutamine into L-alanine and L-glutamine. This slow release of low-concentration L-glutamine into the medium mimics a fed-batch culture strategy. It improves the utilization rate of L-glutamine without generating excess ammonia, thereby creating a more favorable environment for cell growth. L-alanyl-L-glutamine can be used as an equimolar substitute for L-glutamine. It is applicable to nearly all cell types with minimal adaptation required. It also extends cell culture duration, reduces passaging frequency and slows down the decline of cell viability. A slight prolongation of the lag phase may occur after its addition, due to the time required for peptidase secretion and dipeptide hydrolysis, while this effect will not interfere with subsequent cell culture. HEPES is an excellent biological buffer with no cytotoxicity. When supplemented in culture media, it maintains a stable pH range for an extended period and effectively prevents adverse impacts on cell growth caused by drastic pH fluctuations in the medium.
Ingredients contained in this product: D-Glucose, L-alanyl-L-glutamine, Phenol Red, HEPES
Ingredients not contained in this product: L-Glutamine, Sodium Pyruvate
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