BioReagent, 50% v/v BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at 2-8°C,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
This product is composed of a novel high-rigidity agarose matrix coupled with recombinant Protein A (rProtein A) ligand. The rProtein A is expressed and purified from E. coli, ensuring an animal-origin-free production process. The newly designed rProtein A exhibits improved alkali resistance and stronger specific binding to the Fc region of IgG compared to native Protein A. The high-rigidity agarose matrix offers excellent chemical stability and biocompatibility. Through site-specific coupling technology, rProtein A is immobilized onto the matrix, resulting in high binding capacity, high flow rate, superior physicochemical stability, low non-specific adsorption, minimal ligand leakage, and extended service life.
This resin is suitable for one-step purification of high-purity antibodies from samples such as ascites, serum, and cell culture supernatant, making it an ideal choice for large-scale antibody production.
Aladdin Alkali-tolerant rProtein A Agarose Resin is stored in 20% ethanol, with a settled gel to storage solution ratio of 1:1. The product specification refers to the actual volume of the settled gel.
| Parameter | Value |
| Matrix | High-rigidity agarose |
| Ligand | Alkali-resistant rProtein A |
| Average Particle Size | 70 μm |
| Dynamic Binding Capacity (6 min residence time) | 65–80 mg human IgG/mL resin |
| Maximum Flow Rate (25°C) | 300 cm/h |
| Ligand Leakage | ＜10 ng/mL |
| Maximum Pressure Tolerance | 0.3 MPa (3 bar) |
| Cleaning-in-Place (CIP) | 0.1~0.5 M NaOH pH 2~14 |
| Storage Conditions | 2–8°C in 20% ethanol or 2% benzyl alcohol |
Protocol
Due to varying affinity of the resin for antibodies from different species and subtypes, and differences in buffer conditions required to maintain antibody bioactivity, method screening is recommended.
A reference purification procedure is provided below:
1.Equilibration: Equilibrate the column with 5–10 column volumes (CV) of Buffer A (20 mM phosphate buffer + 0.15 M NaCl, pH 7.0; or 0.05 M borate, 4.0 M NaCl, pH 9.0) until the baseline stabilizes.
2.Loading: Load the sample. For solid samples, dissolve in Buffer A. For liquid samples, dialyze against Buffer A prior to loading.
3.Washing: Wash the column with 5 CV of Buffer A until the UV baseline returns to baseline levels.
4.Elution: Elute bound antibodies using a linear gradient over 10 CV to 100% Buffer B (20 mM citrate, pH 3.0; or 0.1 M glycine, pH 3.0; or 20 mM acetate, pH 3.0).
5.Neutralization: Collect the elution fractions and immediately neutralize using a neutralization buffer (e.g., 1.0 M Tris-HCl, pH 9.0, or other suitable buffer) to bring the antibody solution to a stable pH.
6.Regeneration & Cleaning-in-Place (CIP): After multiple cycles, regenerate the column using one of the following methods:
Option 1: Wash with 3–5 CV of 0.1 M acetic acid or 0.1 M acetic acid/20% ethanol. Rinse with buffer until neutral pH is reached before reuse.
Option 2: Wash with 3–5 CV of 0.1–0.5 M NaOH. Rinse with 3–10 CV of purified water. Equilibrate with buffer to neutral pH before reuse.
Comprehensive hazard, handling, storage, and regulatory compliance document.
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